Effect of the oxidizing agents chloramine-T and cigarette smoke on dog serum proteinase inhibitor(s)
Journal Article
·
· Exp. Lung Res.; (United States)
Dog serum treated with the oxidant chloramine-T is rapidly and selectively depleted of its ability to inhibit porcine pancreatic elastase or dog neutrophil elastase. Trypsin inhibitory capacity of serum is not affected. Purified dog alpha-1-proteinase inhibitor (alpha-1-PI) is similarly oxidized with an apparent rate constant of 1.1 x 10(3) M-1 sec-1. Reversal of the oxidative inactivation using dithiothreitol was demonstrated. Cigarette smoke also directly affects the inhibitory capacity of both serum and pure alpha-1-PI. These studies form a basis for developing a model of functionally deficient alpha-1-PI by taking advantage of oxidative inactivation of normal proteinase inhibitor levels.
- OSTI ID:
- 5731506
- Journal Information:
- Exp. Lung Res.; (United States), Journal Name: Exp. Lung Res.; (United States) Vol. 1:3; ISSN EXLRD
- Country of Publication:
- United States
- Language:
- English
Similar Records
Ozone inactivation of human alpha 1-proteinase inhibitor
Genetically engineered, mutant human alpha-1-proteinase inhibitor is more resistant than the normal inhibitor to oxidative inactivation by chemicals, enzymes, cells, and cigarette smoke
Alpha 1-proteinase inhibitor is more sensitive to inactivation by cigarette smoke than is leukocyte elastase
Journal Article
·
Sun Jun 01 00:00:00 EDT 1980
· Am. Rev. Respir. Dis.; (United States)
·
OSTI ID:5940252
Genetically engineered, mutant human alpha-1-proteinase inhibitor is more resistant than the normal inhibitor to oxidative inactivation by chemicals, enzymes, cells, and cigarette smoke
Journal Article
·
Fri Feb 28 23:00:00 EST 1986
· Am. Rev. Respir. Dis.; (United States)
·
OSTI ID:5736936
Alpha 1-proteinase inhibitor is more sensitive to inactivation by cigarette smoke than is leukocyte elastase
Journal Article
·
Fri Oct 01 00:00:00 EDT 1982
· Am. Rev. Respir. Dis.; (United States)
·
OSTI ID:6578457
Related Subjects
560305* -- Chemicals Metabolism & Toxicology-- Vertebrates-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AEROSOLS
AMINES
ANIMALS
BIOLOGICAL EFFECTS
BLOOD SERUM
CHLORAMINES
COLLOIDS
DISPERSIONS
DOGS
ENZYME INHIBITORS
ENZYMES
HYDROLASES
MAMMALS
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
PEPTIDE HYDROLASES
RESIDUES
SMOKES
SOLS
TOBACCO SMOKES
VERTEBRATES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AEROSOLS
AMINES
ANIMALS
BIOLOGICAL EFFECTS
BLOOD SERUM
CHLORAMINES
COLLOIDS
DISPERSIONS
DOGS
ENZYME INHIBITORS
ENZYMES
HYDROLASES
MAMMALS
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
PEPTIDE HYDROLASES
RESIDUES
SMOKES
SOLS
TOBACCO SMOKES
VERTEBRATES