Prereplicative events involving simian virus 40 DNA in permissive cells

Journal Article · Fri Jan 01 00:00:00 EST 1982 · J. Virol.; (United States)

OSTI ID:5730030

Rinaldy, A ^[1]; Feunteun, J; Rosenberg, B H

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1. Sloan-Kettering Inst., Rye, NY

Simian virus 40 DNA molecules were found to be unable to replicate for 9 h after infection, even in cells that were already replicating the DNA of preinfecting simian virus 40; after 9 h, the ability of the DNA to replicate began to rise sharply. The kinetics of activation indicated that each DNA molecule undergoes a series of slow consecutive reactions, not involving T-antigen, before it can replicate. These pre-replicative molecular transformations probably involve configurational changes; their nature and their relation to the initiation of viral DNA synthesis is discussed. Observation of the replicative behavior of one viral DNA in the presence of another was made possible by the use of two different mutants with distinguishable DNAs: a viable deletion mutant containing DNA insensitive to TaqI restriction enzyme was used to provide viral functions required for replication, and is a tsA mutant with TaqI-sensitive DNA was introduced at various times as a probe to determine the ability of the DNA to replicate under different conditions.

• DOE Contract Number: AC02-79EV10263
• OSTI ID: 5730030
• Journal Information: J. Virol.; (United States), Vol. 41:1
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
DNA REPLICATION
BIOCHEMICAL REACTION KINETICS
SIMIAN VIRUS
ANTIGENS
CELL CULTURES
CONFIGURATION INTERACTION
DNA
EXPERIMENTAL DATA
INFECTIVITY
MUTATIONS
DATA
INFORMATION
KINETICS
MICROORGANISMS
NUCLEIC ACID REPLICATION
NUCLEIC ACIDS
NUMERICAL DATA
ORGANIC COMPOUNDS
PARASITES
REACTION KINETICS
VIRUSES
550200* - Biochemistry
550700 - Microbiology