Comparison of the effectiveness of seven amiloride congeners as inhibitors of Na/H and Na/Ca antiport in cultured smooth muscle cells

Conference · Fri May 01 04:00:00 EDT 1987 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:5727409

Smith, L; Higgins, B L; Cragoe, Jr, E J; Smith, J B

The authors cultured smooth muscle cells from rat aorta and assayed Na/Ca antiport by measuring the initial rate of /sup 45/Ca influx in Na-loaded cells. Na/H antiport was assayed by measuring the initial rate of /sup 22/Na influx in acid-loaded cells. The external medium was the same for both assays except Na was 10 mM for Na/H antiport and O for the Na/Ca antiport assay. The dose of each congener that caused 50% inhibition (I/sub 50/) was calculated using a log-log median effect plot. The linear regression coefficients ranged from 0.916 to 0.998. Of all the compounds tested only dimethylbenzamil is more potent as an inhibitor of Na/Ca compared to Na/H antiport.

• Research Organization: Univ. of Alabama, Birmingham
• OSTI ID: 5727409
• Report Number(s): CONF-870644-
• Conference Information: Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 46:6
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALKALI METAL COMPOUNDS
ALKALINE EARTH ISOTOPES
ALKALINE EARTH METAL COMPOUNDS
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CALCIUM 45
CALCIUM COMPOUNDS
CALCIUM ISOTOPES
CATIONS
CELL CULTURES
CHARGED PARTICLES
DAYS LIVING RADIOISOTOPES
DRUGS
EVEN-ODD NUCLEI
HYDROGEN COMPOUNDS
INHIBITION
INTERMEDIATE MASS NUCLEI
IONS
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
MAMMALS
MEMBRANE TRANSPORT
MUSCLES
NUCLEI
RADIOISOTOPES
RATS
RESPONSE MODIFYING FACTORS
RODENTS
SODIUM COMPOUNDS
TRACER TECHNIQUES
VERTEBRATES