Intracellular Na+ regulation of Na+ pump sites in cultured vascular smooth muscle cells

Journal Article · Sat Apr 01 04:00:00 EST 1989 · Am. J. Physiol.; (United States)

OSTI ID:6102072

Allen, J C; Navran, S S; Seidel, C L; Dennison, D K; Amann, J M; Jemelka, S K

Enzymatically dispersed cells from canine saphenous vein and femoral artery were grown in fetal calf serum and studied at day 0 (freshly dispersed) through confluence in primary culture. Intracellular Na levels (Nai), but not intracellular K (Ki), were increased after 24 h in culture and then decreased to a steady state by 4 days. Na+ pump site number (( /sup 3/H) ouabain binding) increased through day 3 and remained elevated. Nai was still elevated at 2 days when the Na+ pump site number began to increase. Total pump turnover (maximum ouabain-inhibited /sup 86/Rb uptake) reflected the increase in Na+ pump site number. These key events precede the observed increases in both protein production and cellular proliferation. If the same cells are maintained in defined medium, without fetal calf serum, Nai, Ki, and the number of (/sup 3/H)ouabain binding sites do not change with time. These data are consistent with the suggestion that the initial mitogenic response of vascular smooth muscle cells to fetal calf serum involves an increased Na+ influx, and a Nai accumulation, caused by low Na+ pump density. The synthesis of new pump sites effects a decrease in the accumulated Nai, which may be related to cell proliferation.

• Research Organization: Baylor College of Medicine, Houston, TX (USA)
• OSTI ID: 6102072
• Journal Information: Am. J. Physiol.; (United States), Journal Name: Am. J. Physiol.; (United States) Vol. 256; ISSN AJPHA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALKALI METAL COMPOUNDS
ALKALI METAL ISOTOPES
ALKALI METALS
ANIMAL CELLS
ANIMALS
ARTERIES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BIOCHEMISTRY
BLOOD VESSELS
BODY
CARBOHYDRATES
CARDIAC GLYCOSIDES
CARDIOTONICS
CARDIOVASCULAR AGENTS
CARDIOVASCULAR SYSTEM
CATIONS
CELL CULTURES
CELL CYCLE
CELL PROLIFERATION
CHARGED PARTICLES
CHEMISTRY
CULTURE MEDIA
DAYS LIVING RADIOISOTOPES
DOGS
DRUGS
ELEMENTS
GLYCOSIDES
INTERMEDIATE MASS NUCLEI
IONS
ISOMERIC TRANSITION ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LABELLED COMPOUNDS
MAMMALS
MEMBRANE PROTEINS
MEMBRANE TRANSPORT
METALS
MINUTES LIVING RADIOISOTOPES
MUSCLES
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANS
OUABAIN
POTASSIUM
PROTEINS
RADIOISOTOPES
REACTION KINETICS
RECEPTORS
RUBIDIUM 86
RUBIDIUM ISOTOPES
SODIUM COMPOUNDS
STROPHANTHINS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VEINS
VERTEBRATES