A preliminary biophysical analysis of site-specific mutations in the photosystem II reaction center of the cyanobacterium Synechocystis 6803
- Michigan State Univ., East Lansing (USA)
In an attempt to identify ligands for the manganese cluster and understand the conformational constraints around Ya{sup +}; we have generated a series of site-specific mutations in the psbA and psbD genes of Synechocystis PCC 6803, specifically at aspartate and glutamate residues in the AB interhelical region of the D1 and D2 polypeptides; and P162D1, P161D2, W167D2 and F168D1 in the vicinity of Yz{sup +}. One of these mutations is lethal and only grows photoheterotrophically in the presence of glucose, and the remainder have an altered oxygen evolution rate but are capable of photoautotrophic growth in the absence of glucose. Preliminary biochemical and spectroscopic data will be presented.
- OSTI ID:
- 5726558
- Report Number(s):
- CONF-9007196--
- Journal Information:
- Plant Physiology, Supplement; (USA), Journal Name: Plant Physiology, Supplement; (USA) Vol. 93:1; ISSN PPYSA; ISSN 0079-2241
- Country of Publication:
- United States
- Language:
- English
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