Targeted mutagenesis of genes for hydrophobic proteins of photosynthetic membranes. [Synechocystis, Rhodopsedomonas viridis]

Conference · Tue Apr 01 00:00:00 EST 1986 · Plant Physiol., Suppl.; (United States)

OSTI ID:5021375

Pakrasi, H; Vermaas, W; Chisholm, D; Williams, J; Arntzen, C

Synechocystis 6803 is a unicellular cyanobacterium that has a photosynthetic system similar to that in higher plants. An efficient DNA-mediated transformation system has been developed for the engineering of membrane proteins in this organisms. S. 6803 can grow photoheterotrophically on glucose when PSII function is inhibited, thereby allowing the recovery of insertion and deletion mutations in six genes encoding polypeptide subunits of the PSII core complex. The chromosome of S. 6803 contains two copies of each of the genes, psbA and psbD, which encode the D1 and D2 proteins of PSII (32 and 34kDa), respectively. All of these genes have been cloned from S. 6803. Currently oligonucleotide-directed site-specific mutagenesis procedures are used to alter amino acids in the D1 and D2 proteins that are predicted to take part in the binding of specific cofactors, e.g., quinones and P680, the reaction center chlorophyll molecule(s). These predictions are based upon the recent structural analysis of the reaction center of Rhodopseudomonas viridis.

• Research Organization: E.I. DuPont de Nemours and Co., Wilmington, DE
• OSTI ID: 5021375
• Journal Information: Plant Physiol., Suppl.; (United States), Vol. 80:4; Conference: Annual meeting of the American Society of Plant Physiologists, Baton Rouge, LA, USA, 8-12 Jun 1986
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CELL MEMBRANES
PROTEINS
CYANOBACTERIA
PHOTOSYNTHESIS
GENES
MUTAGENESIS
AMINO ACIDS
CHLOROPHYLL
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CHEMICAL REACTIONS
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
MEMBRANES
MICROORGANISMS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHOTOCHEMICAL REACTIONS
PHYTOCHROMES
PIGMENTS
PORPHYRINS
SYNTHESIS
550400* - Genetics