Method for /sup 14/C and /sup 3/H double-label autoradiography

Journal Article · Thu Oct 01 04:00:00 EDT 1987 · J. Cereb. Blood Flow Metab.; (United States)

DOI:https://doi.org/10.1038/jcbfm.1987.107· OSTI ID:5725536

Juhler, M; Diemer, N H

The present study describes and validates a /sup 3/H//sup 14/C double-label autoradiographic method in which separation of the labels was obtained by sequential film exposures to film types sensitive to /sup 14/C only and to both /sup 3/H and /sup 14/C, respectively. The error in assuming a pure /sup 14/C image on the first film was 2-3%. A linear subtraction equation was developed for calculation of the /sup 3/H activity expressed in /sup 14/C equivalents as the difference in tissue activity between the second and the first film exposure. The actual /sup 3/H activity in the tissue could be obtained by multiplying the result by 25 (/sup 14/C to /sup 3/H conversion factor). The subtraction procedure was validated for absolute /sup 14/C and /sup 3/H activities of 100-1300 nCi/g and 600-11,000 nCi/g, respectively, and for relative /sup 3/H//sup 14/C activities between 5 and 10. Self-absorption of /sup 3/H in white matter was corrected for by multiplication by 1.61 (self-absorption coefficient). This factor was close to unity for /sup 14/C.

• Research Organization: Rigshospitalet, Copenhagen, Denmark
• OSTI ID: 5725536
• Journal Information: J. Cereb. Blood Flow Metab.; (United States), Journal Name: J. Cereb. Blood Flow Metab.; (United States) Vol. 7:5; ISSN JCBMD
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMALS
AUTORADIOGRAPHY
BODY
BRAIN
CARBON 14 COMPOUNDS
CENTRAL NERVOUS SYSTEM
DOUBLE LABELLING
DUAL-ISOTOPE SUBTRACTION TECHNIQUE
EQUATIONS
ERRORS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LABELLING
MAMMALS
NERVOUS SYSTEM
ORGANS
RATS
RODENTS
TESTING
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VALIDATION
VERTEBRATES