Quantitation of pyrimidine dimer contents of nonradioactive deoxyribonucleic acid by electrophoresis in alkaline agarose gels

Journal Article · Tue Feb 15 04:00:00 EST 1983 · Biochemistry; (United States)

DOI:https://doi.org/10.1021/bi00273a006· OSTI ID:5724315

Sutherland, B M; Shih, A G

We have developed a method of quantitating the pyrimidine dimer content of nonradioactive DNAs. DNA samples are treated with the UV-endonuclease from Micrococcus luteus and then separated according to molecular weight by electrophoresis on alkaline agarose gels. From their migration relative to known molecular weight standards, their median molecular weight and thus the number of dimers per DNA molecule in each sample can be calculated. Results of action spectra for dimer formation in T7 bacteriophage measured by this method agree well with action spectra for T7 killing. In addition, the method gives dimer yields in good agreement with those obtained by others using alkaline sucrose gradient sedimentation.

• Research Organization: Brookhaven National Lab., Upton, NY
• OSTI ID: 5724315
• Journal Information: Biochemistry; (United States), Journal Name: Biochemistry; (United States) Vol. 22:4; ISSN BICHA
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
AZINES
BACTERIA
BACTERIOPHAGES
CHEMICAL ANALYSIS
COLLOIDS
DIMERS
DISPERSIONS
DNA
ELECTROMAGNETIC RADIATION
ELECTROPHORESIS
ENZYMES
ESTERASES
GELS
HETEROCYCLIC COMPOUNDS
HYDROLASES
MICROCOCCUS
MICROCOCCUS LUTEUS
MICROORGANISMS
MOLECULAR WEIGHT
NUCLEASES
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PARASITES
PHOSPHODIESTERASES
PYRIMIDINES
QUANTITATIVE CHEMICAL ANALYSIS
RADIATIONS
SEPARATION PROCESSES
ULTRAVIOLET RADIATION
VIRUSES