Quantitation of pyrimidine dimer contents of nonradioactive deoxyribonucleic acid by electrophoresis in alkaline agarose gels

Journal Article · Tue Feb 15 00:00:00 EST 1983 · Biochemistry; (United States)

DOI:https://doi.org/10.1021/bi00273a006· OSTI ID:5724315

Sutherland, B M; Shih, A G

We have developed a method of quantitating the pyrimidine dimer content of nonradioactive DNAs. DNA samples are treated with the UV-endonuclease from Micrococcus luteus and then separated according to molecular weight by electrophoresis on alkaline agarose gels. From their migration relative to known molecular weight standards, their median molecular weight and thus the number of dimers per DNA molecule in each sample can be calculated. Results of action spectra for dimer formation in T7 bacteriophage measured by this method agree well with action spectra for T7 killing. In addition, the method gives dimer yields in good agreement with those obtained by others using alkaline sucrose gradient sedimentation.

• Research Organization: Brookhaven National Lab., Upton, NY
• OSTI ID: 5724315
• Journal Information: Biochemistry; (United States), Vol. 22:4
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
DNA
ELECTROPHORESIS
BACTERIOPHAGES
DIMERS
GELS
MICROCOCCUS LUTEUS
MOLECULAR WEIGHT
NUCLEASES
PYRIMIDINES
QUANTITATIVE CHEMICAL ANALYSIS
SEPARATION PROCESSES
ULTRAVIOLET RADIATION
AZINES
BACTERIA
CHEMICAL ANALYSIS
COLLOIDS
DISPERSIONS
ELECTROMAGNETIC RADIATION
ENZYMES
ESTERASES
HETEROCYCLIC COMPOUNDS
HYDROLASES
MICROCOCCUS
MICROORGANISMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PARASITES
PHOSPHODIESTERASES
RADIATIONS
VIRUSES
550200* - Biochemistry