The monovalent cation site of pyruvate kinase and other enzymes: NMR investigations

Journal Article · Mon Nov 01 04:00:00 EST 1982 · Fed. Proc.; (United States)

OSTI ID:5724148

Villafranca, J J; Raushel, F M

Several monovalent cations have ideal physical properties for investigation of their interactions with biomacromolecules by NMR techniques. /sup 7/Li/sup +/, /sup 14/NH/sub 4//sup +/ and /sup 133/Cs/sup +/ have high natural abundance, and enriched samples of /sup 6/Li/sup +/ and /sup 15/NH/sub 4//sup +/ are easily obtained. All of these nuclei share the common property of having narrow line widths, thereby providing high sensitivity for NMR experiments. Numerous /sup 23/Na/sup +/ NMR studies have been reported but /sup 39/K/sup +/, /sup 85/Rb/sup +/, and /sup 87/Rb/sup +/ have not been studied extensively because these nuclei have low sensitivity and broad line widths. Recently NMR studies of monovalent cations have been extended to explore the monovalent cation sites of enzymes activated by such cations. With pyruvate kinase we determined that the extent of activation elicited by monovalent cations correlated well with the distance between the monovalent and divalent cations (structural site) sites. Those ions that were the best activators were closest to the M/sup 2 +/ site. In the course of this investigation we developed a general method to determine correlation times for dipolar relaxation between monovalent cation sites and paramagnetic centers on enzymes, and we have extended this work to other enzymes that are activated by monovalent cations.

• Research Organization: Pennsylvania State Univ., University Park
• OSTI ID: 5724148
• Journal Information: Fed. Proc.; (United States), Journal Name: Fed. Proc.; (United States) Vol. 41:13; ISSN FEPRA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALKALI METAL COMPOUNDS
ALKALI METAL ISOTOPES
ALKALI METALS
AMMONIUM COMPOUNDS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOXYLIC ACIDS
CATIONS
CESIUM 133
CESIUM COMPOUNDS
CESIUM ISOTOPES
CHARGED PARTICLES
CHEMICAL ACTIVATION
CHEMICAL PROPERTIES
ELEMENTS
ENZYMES
INTERMEDIATE MASS NUCLEI
IONS
ISOTOPES
KETO ACIDS
LIGHT NUCLEI
LITHIUM 6
LITHIUM 7
LITHIUM COMPOUNDS
LITHIUM ISOTOPES
MAGNETIC RESONANCE
METALS
NITROGEN 14
NITROGEN 15
NITROGEN ISOTOPES
NUCLEAR MAGNETIC RESONANCE
NUCLEI
ODD-EVEN NUCLEI
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHOTRANSFERASES
PHYSICAL PROPERTIES
POTASSIUM 39
POTASSIUM COMPOUNDS
POTASSIUM ISOTOPES
PYRUVIC ACID
RADIOISOTOPES
RESONANCE
RUBIDIUM 85
RUBIDIUM 87
RUBIDIUM COMPOUNDS
RUBIDIUM ISOTOPES
SODIUM 23
SODIUM COMPOUNDS
SODIUM ISOTOPES
STABLE ISOTOPES
STRUCTURAL CHEMICAL ANALYSIS
TRANSFERASES
VALENCE
YEARS LIVING RADIOISOTOPES