Microbial fermentative preparation of L-(/sup 15/N/sub 2/)lysine and its tracer: application to serum amino acid kinetic studies
Journal Article
·
· Anal. Biochem.; (United States)
OSTI ID:5723315
The microorganism Brevibacterium flavium 21129 has been used to produce multigram batches of L-(/sup 15/N/sub 2/)lysine of high purity and isotopic enrichment by supplementation of the growth medium with (/sup 15/NH/sub 4/)/sub 2/SO/sub 4/ of 98.0 atom% excess. The doubly /sup 15/N-labeled lysine can be detected at dilutions 10 times greater than singly labeled lysine when isotope dilution curves are analyzed by gas chromatography-mass spectrometry. This enhanced sensitivity permits kinetic measurements of plasma free-lysine isotope content over a 300-fold dilution during 6 h following a single oral bolus of 5 mg/kg body wt. This inexpensive preparation method lends itself to the production of highly useful biochemical compounds for kinetic studies of human nutrition.
- Research Organization:
- Baylor College of Medicine, Houston, TX
- DOE Contract Number:
- W-31109-ENG-38
- OSTI ID:
- 5723315
- Journal Information:
- Anal. Biochem.; (United States), Journal Name: Anal. Biochem.; (United States) Vol. 131:1; ISSN ANBCA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
09 BIOMASS FUELS
140504 -- Solar Energy Conversion-- Biomass Production & Conversion-- (-1989)
550701* -- Microbiology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
BACTERIA
BIOCHEMICAL REACTION KINETICS
BIOCONVERSION
BIOSYNTHESIS
CARBOXYLIC ACIDS
CHROMATOGRAPHY
DOUBLE LABELLING
FERMENTATION
GAS CHROMATOGRAPHY
ISOTOPE APPLICATIONS
ISOTOPE DILUTION
ISOTOPES
KINETICS
LABELLED COMPOUNDS
LABELLING
LIGHT NUCLEI
LYSINE
MASS SPECTROSCOPY
MICROORGANISMS
NITROGEN 15
NITROGEN ISOTOPES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
REACTION KINETICS
SEPARATION PROCESSES
SPECTROSCOPY
STABLE ISOTOPES
SYNTHESIS
TRACER TECHNIQUES
USES
140504 -- Solar Energy Conversion-- Biomass Production & Conversion-- (-1989)
550701* -- Microbiology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
BACTERIA
BIOCHEMICAL REACTION KINETICS
BIOCONVERSION
BIOSYNTHESIS
CARBOXYLIC ACIDS
CHROMATOGRAPHY
DOUBLE LABELLING
FERMENTATION
GAS CHROMATOGRAPHY
ISOTOPE APPLICATIONS
ISOTOPE DILUTION
ISOTOPES
KINETICS
LABELLED COMPOUNDS
LABELLING
LIGHT NUCLEI
LYSINE
MASS SPECTROSCOPY
MICROORGANISMS
NITROGEN 15
NITROGEN ISOTOPES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
REACTION KINETICS
SEPARATION PROCESSES
SPECTROSCOPY
STABLE ISOTOPES
SYNTHESIS
TRACER TECHNIQUES
USES