Dialdehyde derivatives of purine mononucleotides: substrate properties and affinity modification of myosin ATPase
Journal Article
·
· Biochemistry (Engl. Transl.); (United States)
OSTI ID:5722628
It was established that the dialdehyde derivative of ATP (oxo-ATP) is a good substrate of the Ca-ATPase of heavy meromyosin: (1.2-1.4) x 10/sup -4/ M; V = V/sub ATP/. At the same time, it is capable of inducing irreversible inhibition of the enzyme. Since oxo-ATP is rapidly digested by myosin, forming oxo-ADP, this inhibition is a consequence of the interaction of the enzyme with oxo-ADP. It was shown that the inhibition of heavy meromyosin (HMM), by oxo-ADP occurs according to the kinetics characteristic of affinity modification; moreover, ATP entirely protects HMM from the loss of activity. Similar data on the irreversible inhibition of ATPase activity under the action of oxo-ADP were obtained in the case of myosin, heavy meromyosin, subfragment-1, and natural actomyosin, as well as in the absence of divalent cations, which is evidence of modification of the active site of myosin ATPase.
- Research Organization:
- M.V. Lomonosov Moscow State Univ., USSR
- OSTI ID:
- 5722628
- Journal Information:
- Biochemistry (Engl. Transl.); (United States), Journal Name: Biochemistry (Engl. Transl.); (United States) Vol. 50:9; ISSN BIORA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ACID ANHYDRASES
ADP
ALDEHYDES
ANIMALS
AROMATICS
ATP
ATP-ASE
AZAARENES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMISTRY
CHEMISTRY
COUNTING TECHNIQUES
DAYS LIVING RADIOISOTOPES
ENZYME ACTIVITY
ENZYME INHIBITORS
ENZYMES
GLOBULINS
HETEROCYCLIC COMPOUNDS
HYDROLASES
INHIBITION
IRREVERSIBLE PROCESSES
ISOTOPES
LABELLED COMPOUNDS
LABELLING
LIGHT NUCLEI
MAMMALS
MYOSIN
NUCLEI
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHOSPHOHYDROLASES
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PROTEINS
PURINES
RADIOENZYMATIC ASSAY
RADIOISOTOPES
RATS
RODENTS
SCINTILLATION COUNTING
SUBSTRATES
VERTEBRATES
59 BASIC BIOLOGICAL SCIENCES
ACID ANHYDRASES
ADP
ALDEHYDES
ANIMALS
AROMATICS
ATP
ATP-ASE
AZAARENES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMISTRY
CHEMISTRY
COUNTING TECHNIQUES
DAYS LIVING RADIOISOTOPES
ENZYME ACTIVITY
ENZYME INHIBITORS
ENZYMES
GLOBULINS
HETEROCYCLIC COMPOUNDS
HYDROLASES
INHIBITION
IRREVERSIBLE PROCESSES
ISOTOPES
LABELLED COMPOUNDS
LABELLING
LIGHT NUCLEI
MAMMALS
MYOSIN
NUCLEI
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHOSPHOHYDROLASES
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PROTEINS
PURINES
RADIOENZYMATIC ASSAY
RADIOISOTOPES
RATS
RODENTS
SCINTILLATION COUNTING
SUBSTRATES
VERTEBRATES