Direct photoaffinity labeling by nucleotides of the apparent catalytic site on the heavy chains of smooth muscle and Acanthamoeba myosins
Journal Article
·
· J. Biol. Chem.; (United States)
OSTI ID:6172038
The heavy chains of Acanthamoeba myosins, IA, IB and II, turkey gizzard myosin, and rabbit skeletal muscle myosin subfragment-1 were specifically labeled by radioactive ATP, ADP, and UTP, each of which is a substrate or product of myosin ATPase activity, when irradiated with uv light at 0/sup 0/C. With UTP, as much as 0.45 mol/mol of Acanthamoeba myosin IA heavy chain and 1 mol/mol of turkey gizzard myosin heavy chain was incorporated. Evidence that the ligands were associated with the catalytic site included the observations that reaction occurred only with nucleotides that are substrates or products of the ATPase activity; that the reaction was blocked by pyrophosphate which is an inhibitor of the ATPase activity; that ATP was bound as ADP; and that label was probably restricted to a single peptide following limited subtilisin proteolysis of labeled Acanthamoeba myosin IA heavy chain and extensive cleavage with CNBr and trypsin of labeled turkey gizzard myosin heavy chain.
- OSTI ID:
- 6172038
- Journal Information:
- J. Biol. Chem.; (United States), Journal Name: J. Biol. Chem.; (United States) Vol. 256:1; ISSN JBCHA
- Country of Publication:
- United States
- Language:
- English
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Conference
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Thu May 01 00:00:00 EDT 1986
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OSTI ID:6905001
Direct photoaffinity labeling of gizzard myosin with ( sup 3 H)uridine diphosphate places Glu185 of the heavy chain at the active site
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Related Subjects
550200 -- Biochemistry
560111* -- Radiation Effects on Biochemicals-- In Vitro-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACID ANHYDRASES
ADP
AMOEBA
AMP
ANIMALS
ATP
ATP-ASE
BIRDS
CHEMICAL REACTIONS
ELECTROMAGNETIC RADIATION
ENZYME ACTIVITY
ENZYME INHIBITORS
ENZYMES
FOWL
GLOBULINS
HYDROLASES
INVERTEBRATES
LABELLING
MAMMALS
METABOLISM
MICROORGANISMS
MUSCLES
MYOSIN
NUCLEOTIDES
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
PEPTIDE HYDROLASES
PEPTIDES
PHOSPHOHYDROLASES
PHOSPHORUS COMPOUNDS
PHOTOCHEMICAL REACTIONS
PROTEINS
PROTOZOA
PYROPHOSPHATES
RABBITS
RADIATIONS
SARCODINA
SERINE PROTEINASES
SUBSTRATES
TRYPSIN
ULTRAVIOLET RADIATION
VERTEBRATES
560111* -- Radiation Effects on Biochemicals-- In Vitro-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACID ANHYDRASES
ADP
AMOEBA
AMP
ANIMALS
ATP
ATP-ASE
BIRDS
CHEMICAL REACTIONS
ELECTROMAGNETIC RADIATION
ENZYME ACTIVITY
ENZYME INHIBITORS
ENZYMES
FOWL
GLOBULINS
HYDROLASES
INVERTEBRATES
LABELLING
MAMMALS
METABOLISM
MICROORGANISMS
MUSCLES
MYOSIN
NUCLEOTIDES
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
PEPTIDE HYDROLASES
PEPTIDES
PHOSPHOHYDROLASES
PHOSPHORUS COMPOUNDS
PHOTOCHEMICAL REACTIONS
PROTEINS
PROTOZOA
PYROPHOSPHATES
RABBITS
RADIATIONS
SARCODINA
SERINE PROTEINASES
SUBSTRATES
TRYPSIN
ULTRAVIOLET RADIATION
VERTEBRATES