Study of the conditions of activation and stabilization of Shigella sonnei 47 DNA methylases in the process of fractionation, purification, and during storage

Journal Article · Fri Feb 20 04:00:00 EST 1987 · Biochemistry (Engl. Transl.); (United States)

OSTI ID:5721910

Suchkov, S V; Lopatina, N G; Arutyunyan, E E; Nikol'skaya, I I; Debov, S S

A comparative investigation was made of the factors of activation and conditions of stabilization of partially purified and individual fractions of DNA methylases of the strain Shigella sonnei 47. The influence of the temperature system, glycerin, albumin, protease inhibitors, divalent metal ions, as well as the conditions of storage at the value of the isoelectric point of the protein on the activity of the enzymes of methylation was studied. It was shown that the effects of activating factors and levels of stabilization differ appreciably, depending on the degree of purification and the composition of the enzyme preparations. It was established that glycerin is ineffective as a stabilizing agent. It was shown that of all the divalent cations studied the only activator universal for the methylases of Shigella sonnei 47 is Ca/sup 2 +/ ions. A pronounced stabilizing effect of albumin on the activity of these DNA methylases was demonstrated. With the exception of one enzymatic fraction, the protease inhibitors have virtually no effect on the level of methylating activity of the preparations. The phenomenon of spontaneous fluctuations of the methylating activity of Shigella sonnei 47 enzyme preparations during storage is described.

• Research Organization: Scientific-Research Institute of Medical Enzymology, Moscow, USSR
• OSTI ID: 5721910
• Journal Information: Biochemistry (Engl. Transl.); (United States), Journal Name: Biochemistry (Engl. Transl.); (United States) Vol. 51:8; ISSN BIORA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
ALKALI METAL COMPOUNDS
ALKALINE EARTH METAL COMPOUNDS
BACTERIA
BIOLOGICAL EFFECTS
BUFFERS
CATIONS
CHARGED PARTICLES
CHEMICAL REACTIONS
COBALT COMPOUNDS
DNA
ENZYME ACTIVITY
ENZYME INHIBITORS
ENZYMES
FRACTIONATION
IONS
LABELLED COMPOUNDS
LABELLING
MAGNESIUM COMPOUNDS
MANGANESE COMPOUNDS
METABOLIC ACTIVATION
METHYL TRANSFERASES
METHYLATION
MICROORGANISMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
POTASSIUM COMPOUNDS
PROTEINS
PURIFICATION
RADIOENZYMATIC ASSAY
SEPARATION PROCESSES
SHIGELLA
SODIUM COMPOUNDS
STABILIZATION
STORAGE LIFE
TEMPERATURE EFFECTS
TRANSFERASES
TRANSITION ELEMENT COMPOUNDS
TRITIUM COMPOUNDS
ZINC COMPOUNDS