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Physical-chemical model for cellular uptake of fatty acids: prediction of intracellular pool sizes

Journal Article · · Biochemistry; (United States)
DOI:https://doi.org/10.1021/bi00392a047· OSTI ID:5721214
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If the uptake of fatty acids by liver is a physical, not a biological, process, then the size and location of the intrahepatic pool of fatty acids can be predicted from uptake rates and thermodynamic data. The purpose of the experiments in this paper was to test the accuracy of this idea. Rat livers were perfused with (/sup 3/H) palmitate bound to (/sup 14/C) albumin, and the total amounts of palmitate removed from the perfusate were measured at 3-s intervals. The intrahepatic pools of palmitate calculated from these data were 13.8 and 23.0 nmol/g of liver at ratios of palmitate/albumin (mol/mol) (afferent side) of 2/1 and 4/1, respectively, in the steady state. The intrahepatic pools of palmitate calculated from the distributions of palmitate between membranes, H/sub 2/O, albumin, and fatty acid binding protein and the measured first-order rate constants for acyl-CoA ligases in mitochondria and microsomes were 12.1 and 34.6 nmol/g for perfusate ratios of palmitate/albumin of 2/1 and 4/1, in the steady state. Intrahepatic pools of palmitate measured after establishment of a steady-state rate of uptake were 15.0 and 31.8 nmol/g for these ratios of palmitate/albumin of 2/1 and 4/1.
Research Organization:
Cornell Univ. Medical College, New York, NY
OSTI ID:
5721214
Journal Information:
Biochemistry; (United States), Journal Name: Biochemistry; (United States) Vol. 26:18; ISSN BICHA
Country of Publication:
United States
Language:
English

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Related Subjects

550201 -- Biochemistry-- Tracer Techniques
550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
ALBUMINS
ANIMAL TISSUES
ANIMALS
BIOCHEMICAL REACTION KINETICS
BODY
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CELL MEMBRANES
DIGESTIVE SYSTEM
GLANDS
HEXADECANOIC ACID
ISOTOPE APPLICATIONS
KINETICS
LABELLED POOL TECHNIQUES
LIVER
MAMMALS
MATHEMATICAL MODELS
MEMBRANES
MONOCARBOXYLIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PERFUSED TISSUES
PROTEINS
RATS
REACTION KINETICS
RODENTS
TISSUES
TRACER TECHNIQUES
UPTAKE
VERTEBRATES