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NMR studies on an oligodeoxynucleotide containing 2-aminopurine opposite adenine

Journal Article · · Biochemistry; (United States)
DOI:https://doi.org/10.1021/bi00392a009· OSTI ID:5721191
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A heteroduplex containing the mismatch 2-aminopurine (AP)-adenine has been synthesized and studied by proton NMR. The mismatch was incorporated into the sequence d(CGG(AP)GGC) x d-(GCCACCG). One-dimensional nuclear Overhauser effect measurements in H/sub 2/O and two-dimensional nuclear Overhauser effect spectra in D/sub 2/O show AP x A base pairs in a wobble structure in which both bases are in the anti conformation. The adenine is stacked well in the helix, but the helix twist between the adenine and neighboring cytosine in the 3' direction is unusually small. As a result, the aminopurine on the opposite strand is somewhat pushed out of the helix. From the measurements of the imino proton line widths, the two adjacent G x C base pairs are not found to be significantly destabilized by the presence of the purine-purine wobble pair.

Research Organization:
Centre d'Etudes Nucleaires de Saclay, Gif-sur-Yvette, France
OSTI ID:
5721191
Journal Information:
Biochemistry; (United States), Journal Name: Biochemistry; (United States) Vol. 26:18; ISSN BICHA
Country of Publication:
United States
Language:
English

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Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ADENINES
AMINES
ANTIMETABOLITES
AROMATICS
AZAARENES
BARYONS
DRUGS
ELEMENTARY PARTICLES
FERMIONS
HADRONS
HEAVY WATER
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
MAGNETIC RESONANCE
MOLECULAR STRUCTURE
NUCLEAR MAGNETIC RESONANCE
NUCLEIC ACIDS
NUCLEONS
OLIGONUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OVERHAUSER EFFECT
OXYGEN COMPOUNDS
PROTONS
PURINES
RESONANCE
WATER