NMR studies of the stable mismatch purine-thymine in the self-complementary d(CGPuAATTTCG) duplex in solution
Journal Article
·
· Biochemistry; (United States)
One- and two-dimensional nuclear Overhauser effect experiments demonstrate that a single hydrogen bond between a T imino proton and purine N3 is sufficient to hold the base pair dPu-dT in d(CGPuAATTTCG) by a Watson-Crick fashion rather than a Hoogsteen type. In addition, the dPu-dT base pair is well stacked with neighboring base pairs. The spin-lattice relaxation measurements at 30 and 35/sup 0/C of two decamers, d(CGPuAATTTCG) and d(CGAAATTTCG), reveal that the elimination of two single hydrogen bonds of dA-dT base pairs (due to the substitution of adenine for purine) in the sequence results in an increase in the overall imino proton exchange rate from 7 to 36 s/sup -1/ at the site of mismatch.
- Research Organization:
- Illinois Institute of Technology, Chicago
- OSTI ID:
- 5878260
- Journal Information:
- Biochemistry; (United States), Journal Name: Biochemistry; (United States) Vol. 26:18; ISSN BICHA
- Country of Publication:
- United States
- Language:
- English
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OSTI ID:1012313
NMR and molecular modeling evidence for a Gter dot A mismatch base pair in a purine-rich DNA duplex
Journal Article
·
Mon Dec 31 23:00:00 EST 1990
· Proceedings of the National Academy of Sciences of the United States of America; (United States)
·
OSTI ID:5012927
NMR studies of echinomycin bisintercalation complexes with d(A1-C2-G3-T4) and d(T1-C2-G3-A4) duplexes in aqueous solution: sequence-dependent formation of Hoogsteen A1 x T4 and Watson-Crick T1 x A4 base pairs flanking the bisintercalation site
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OSTI ID:5381102
Related Subjects
550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
AROMATICS
AZAARENES
AZINES
BARYONS
CHEMICAL BONDS
ELEMENTARY PARTICLES
FERMIONS
HADRONS
HEAVY WATER
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
HYDROXY COMPOUNDS
MAGNETIC RESONANCE
NUCLEAR MAGNETIC RESONANCE
NUCLEIC ACIDS
NUCLEONS
OLIGONUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OVERHAUSER EFFECT
OXYGEN COMPOUNDS
PROTONS
PURINES
PYRIMIDINES
RELAXATION
RESONANCE
SPIN-LATTICE RELAXATION
TEMPERATURE DEPENDENCE
THYMINE
URACILS
WATER
62 RADIOLOGY AND NUCLEAR MEDICINE
AROMATICS
AZAARENES
AZINES
BARYONS
CHEMICAL BONDS
ELEMENTARY PARTICLES
FERMIONS
HADRONS
HEAVY WATER
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
HYDROXY COMPOUNDS
MAGNETIC RESONANCE
NUCLEAR MAGNETIC RESONANCE
NUCLEIC ACIDS
NUCLEONS
OLIGONUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OVERHAUSER EFFECT
OXYGEN COMPOUNDS
PROTONS
PURINES
PYRIMIDINES
RELAXATION
RESONANCE
SPIN-LATTICE RELAXATION
TEMPERATURE DEPENDENCE
THYMINE
URACILS
WATER