Effect of sterol metabolism in the yeast-Drosophila system on the frequency of radiation-induced aneuploidy in the Drosophila melanogaster oocytes
The effect of sterol metabolism on induced mutagenesis of Drosophila melanogaster was studied in the ecogenetic system of yeast-Drosophila. Sterol deficiency was created in Drosophila by using the biomass of live cells of Saccharomyces cerevisiae strain 9-2-P712 till mutation in locus nys/sup r1/ blocking the synthesis of ergosterol as the food. It was found that rearing of Drosophila females on the mutant yeast increases the frequency of loss and nondisjunction of X chromosomes induced in mature oocytes by X rays (1000 R). Addition of 0.1% of cholesterol solution in 10% ethanol to the yeast biomass restores the resistance of oocyte to X irradiation to the control level. The possible hormonal effect on membrane leading to increased radiation-induced aneuploidy in Drosophila and the role of sterol metabolism in determining the resistance to various damaging factors are discussed.
- Research Organization:
- A. A. Zhdanov Leningrad State Univ., USSR
- OSTI ID:
- 5719620
- Journal Information:
- Sov. Genet. (Engl. Transl.); (United States), Vol. 21:7; Other Information: Translated from Genetika; 21: No. 7, 1135-1142(Jul 1985)
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
DROSOPHILA
ANEUPLOIDY
BIOLOGICAL RADIATION EFFECTS
ERGOSTEROL
BIOSYNTHESIS
METABOLISM
OOCYTES
YEASTS
MUTAGENESIS
CHOLESTEROL
CHROMOSOMAL ABERRATIONS
CHROMOSOME LOSSES
ETHANOL
GENETICS
MUTANTS
NON-DISJUNCTION
SACCHAROMYCES CEREVISIAE
X CHROMOSOME
X RADIATION
ALCOHOLS
ANIMALS
ARTHROPODS
BIOLOGICAL EFFECTS
BIOLOGY
CHROMOSOMES
DIPTERA
ELECTROMAGNETIC RADIATION
FLIES
FRUIT FLIES
FUNGI
GERM CELLS
HETEROCHROMOSOMES
HYDROXY COMPOUNDS
INSECTS
INVERTEBRATES
IONIZING RADIATIONS
LOSSES
MICROORGANISMS
MUTATIONS
ORGANIC COMPOUNDS
PLANTS
PLOIDY
RADIATION EFFECTS
RADIATIONS
SACCHAROMYCES
STEROIDS
STEROLS
SYNTHESIS
560152* - Radiation Effects on Animals- Animals
550400 - Genetics
550500 - Metabolism