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Stimulation of cell proliferation and polyphosphoinositide turnover in a yeast sterol auxotroph by ergosterol

Conference · · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
OSTI ID:7025065
; ;

The effect of ergosterol on cell division and phospholipid metabolism was investigated in strain GL7, a sterol auxotroph of Saccharmyces cerevisiae. When starved for ergosterol GL7 remains viable but grows very slowly and partially accumulates in the unbudded state. The readdition of ergosterol alone or together with cholesterol but not cholesterol alone stimulates both budding and cell proliferation noticeably after a lag of several hours. Within 10 min after ergosterol readdition to prelabeled cholesterol-grown cells the /sup 32/P and (/sup 3/H) inositol content of the polyphosphoinositides increases markedly followed by an equally striking and rapid decrease. The phosphatidylinositol kinase activity in the membrane fraction also increases when cells growing on cholesterol were supplemented with a small amount of ergosterol. A serine specific casein kinase activity which is immunoprecipitable by antibody prepared against the Rous sarcoma virus transforming protein, pp60/sup v-src/, is enhanced in ergosterol-grown cells when compared to the kinase activity in cholesterol cells. Taken together the results suggest that increased poly-phosphoinositide turnover and protein phosphorylation are associated with or parallel the stimulation of cell proliferation by a small amount of the yeast's natural sterol.

Research Organization:
Harvard Univ., Cambridge, MA
OSTI ID:
7025065
Report Number(s):
CONF-8606151-
Journal Information:
Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Vol. 45:6; ISSN FEPRA
Country of Publication:
United States
Language:
English

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Related Subjects

550201* -- Biochemistry-- Tracer Techniques
550701 -- Microbiology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
CARBOHYDRATES
CELL PROLIFERATION
CHOLESTEROL
DAYS LIVING RADIOISOTOPES
ENZYME ACTIVITY
ENZYMES
ERGOSTEROL
ESTERS
FUNGI
GROWTH
HYDROXY COMPOUNDS
INOSITOL
INOSITOLS
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
LIPIDS
METABOLISM
MICROORGANISMS
MONOSACCHARIDES
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PHOSPHOLIPIDS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHOTRANSFERASES
PLANTS
RADIOISOTOPES
SACCHARIDES
SACCHAROMYCES
SACCHAROMYCES CEREVISIAE
STEROIDS
STEROLS
TRACER TECHNIQUES
TRANSFERASES
TRITIUM COMPOUNDS
YEASTS