A metal-ion NMR investigation of the antibiotic facilitated transport of monovalent cations through the walls of phospholipid vesicles. II. Sulfur-33 NMR
A technique has been developed to investigate the antibiotic facilitated transmembrane transport of monovalent cations using {sup 23}Na and {sup 7}Li Nuclear Magnetic Resonance spectroscopy. The initial portion of this thesis outlines the production and characterization of a model lipid system amenable to the NMR detection of cation transport. Large unilamellar vesicles (LUV) have been prepared from a 4:1 mixture of phosphatidylcholine and phosphatidylglycerol. The presence of the anionic chemical shift reagent dysprosium (III) tripolyphosphate, either inside or outside of the vesicles, allows for the spectroscopic separation of the NMR resonances arising from the inter- and extravesicular cation pools. The cation transporting properties of the channel-forming pentadecapeptide, gramicidin D, have been studied using the NMR technique.
- Research Organization:
- Arkansas Univ., Fayetteville, AR (USA)
- OSTI ID:
- 5719011
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
ALKALI METAL ISOTOPES
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
CATIONS
CHARGED PARTICLES
CHEMICAL COMPOSITION
CHEMICAL PROPERTIES
CHEMICAL SHIFT
DRUGS
ESTERS
EVEN-ODD NUCLEI
IONS
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
LIPIDS
LITHIUM 7
LITHIUM ISOTOPES
MAGNETIC RESONANCE
MEMBRANE TRANSPORT
NMR SPECTRA
NUCLEAR MAGNETIC RESONANCE
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PHOSPHOLIPIDS
REAGENTS
RESONANCE
SODIUM 23
SODIUM ISOTOPES
SPECTRA
STABLE ISOTOPES
SULFUR 33
SULFUR ISOTOPES
TRACER TECHNIQUES