The mechanism of the transient depression of the erythropoietic rate induced in the rat by a single injection of uranyl nitrate

Journal Article · Thu Jun 15 04:00:00 EDT 1989 · Toxicol. Appl. Pharmacol.; (United States)

DOI:https://doi.org/10.1016/0041-008X(89)90008-2· OSTI ID:5714822

Giglio, M J; Brandan, N; Leal, T L; Bozzini, C E

With the purpose of assessing the effect of uranyl nitrate (UN) on the rate of erythropoiesis, 1 mg/kg of the compound was injected iv to adult female Wistar rats. The dosing vehicle was injected into control animals. A single injection of UN induced a transient depression of the rate of red cell volume /sup 59/Fe uptake, which reached its lowest value (68% depression) by the seventh postinjection day. By 14 days, /sup 59/Fe incorporation had returned to normal. The amount of iron going to erythroid tissue per hour, reticulocyte count, and immunoreactive erythropoietin concentration in both plasma and kidney extracts were also significantly depressed in UN-treated rats in relation to these values in vehicle-injected rats by the seventh postinjection day. Dose-response curves for exogenous erythropoietin (Epo) performed in polycythemic intact and UN-treated rats 7 days after drug injection revealed a significant depression of the response in UN-injected animals. Moreover, bone marrow cells obtained from rats pretreated with UN formed a reduced number of erythroid colonies in vitro in response to Epo. Therefore, possible mechanisms for the observed transient depression in the rate of erythropoiesis associated with acute UN treatment include decreased Epo production and direct or indirect damage of erythroid progenitor cells.

• Research Organization: Univ. of Buenos Aires (Argentina)
• OSTI ID: 5714822
• Journal Information: Toxicol. Appl. Pharmacol.; (United States), Journal Name: Toxicol. Appl. Pharmacol.; (United States) Vol. 99:2; ISSN TXAPA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACTINIDE COMPOUNDS
ACTINIDES
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOSYNTHESIS
BLOOD FORMATION
BODY
BONE MARROW CELLS
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
DOSE-RESPONSE RELATIONSHIPS
ELEMENTS
ERYTHROPOIESIS
ERYTHROPOIETIN
EVEN-ODD NUCLEI
INHIBITION
INJECTION
INTAKE
INTERMEDIATE MASS NUCLEI
INTRAVENOUS INJECTION
IRON 59
IRON ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KIDNEYS
MAMMALS
METALS
MITOGENS
NITRATES
NITROGEN COMPOUNDS
NUCLEI
ORGANIC COMPOUNDS
ORGANS
OXYGEN COMPOUNDS
PROTEINS
RADIOISOTOPES
RATS
RODENTS
SOMATIC CELLS
STEM CELLS
SYNTHESIS
TOXICITY
TRACER TECHNIQUES
URANIUM
URANIUM COMPOUNDS
URANYL COMPOUNDS
URANYL NITRATES
VERTEBRATES