Isolation of novel cDNA transformation markers from SV40-transformed human keratinocytes

Journal Article · Thu Jun 01 04:00:00 EDT 1989 · Experimental Cell Research; (United States)

DOI:https://doi.org/10.1016/0014-4827(89)90250-4· OSTI ID:5714711

Morris, A E ^[1]; Steinberg, M L ^[2]; Le, Jun-Yi; Defendi, V ^[3]

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1. Columbia Univ., New York, NY (United States)
2. City Univ. of New York, NY (United States)
3. New York Univ. Medical Center, New York (United States)

Differential screening of a cDNA library was used to isolate probes from mRNAs that are induced in simian virus 40 (SV40)-transformed human keratinocytes. Several of these cDNAs hybrid select mRNAs which encode transformation-induced proteins found in the cytoskeletal component of SV40-transformed keratinocytes. One of these cDNAs was used to study the phenotype of normal and transformed cell lines derived from various tissues. The authors found that mRNA encoding the novel transformation-induced proteins is expressed in two squamous carcinoma cell lines, and two SV-40 transformed fibroblasts. Normal or transformed lymphoid cells or cell lines derived from carcinoma of the cervix do not express mRNAs which hybridize to these probes. The results from this study suggest that these probes may be used to detect markers of transformation in certain cell types.

• OSTI ID: 5714711
• Journal Information: Experimental Cell Research; (United States), Journal Name: Experimental Cell Research; (United States) Vol. 182:2; ISSN ECREA; ISSN 0014-4827
• Country of Publication: United States
• Language: English

Related Subjects

550301* -- Cytology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MARKERS
CARCINOMAS
CELL TRANSFORMATIONS
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
DISEASES
DNA
DNA HYBRIDIZATION
DNA SEQUENCING
ELECTROPHORESIS
FIBROBLASTS
HYBRIDIZATION
ISOTOPES
LIGHT NUCLEI
MESSENGER-RNA
MICROORGANISMS
NEOPLASMS
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ONCOGENIC TRANSFORMATIONS
ONCOGENIC VIRUSES
ORGANIC COMPOUNDS
PARASITES
PHENOTYPE
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PROTEINS
RADIOISOTOPES
RECOMBINANT DNA
RNA
SOMATIC CELLS
STRUCTURAL CHEMICAL ANALYSIS
TUMOR CELLS
VIRUSES