Expression of membrane glycoproteins in normal keratinocytes and squamous carcinoma cell lines

Journal Article · Tue Aug 01 04:00:00 EDT 1989 · Experimental Cell Research; (United States)

DOI:https://doi.org/10.1016/0014-4827(89)90403-5· OSTI ID:5533807

Rayter, Z ^[1]; McIlhinney, R ^[2]; Gusterson, B ^[3]

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1. St. George's Hospital, London (United Kingdom)
2. The University, Oxford (United Kingdom)
3. Haddow Laboratories, Surrey (United Kingdom)

Con A acceptor glycoproteins were analyzed by 2D-PAGE and {sup 125}I-Con A overlay in three squamous carcinoma cell lines and compared with those in the simian virus (SV40)-transformed keratinocyte cell line SVK-14 and in normal keratinocytes. The majority of the glycoproteins identified by this technique were expressed at similar levels in all of the cells examined, independent of the culture conditions used. A cell surface glycoprotein gp34 was increased in the tumor cells compared with normal keratinocytes and expression varied with the culture density. Another glycoprotein, gp21, was found to be increased in expression in normal keratinocytes and stratified hyperconfluent cultures of squamous carcinoma cell lines. This paper describes the potential of this technique to identify membrane glycoproteins which may be expressed as a function of proliferation or differentiation.

• OSTI ID: 5533807
• Journal Information: Experimental Cell Research; (United States), Journal Name: Experimental Cell Research; (United States) Vol. 183:2; ISSN ECREA; ISSN 0014-4827
• Country of Publication: United States
• Language: English

Related Subjects

550301* -- Cytology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AGGLUTININS
ANIMAL CELLS
ANTIBODIES
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
CARCINOMAS
CELL CONSTITUENTS
CELL DIFFERENTIATION
CELL MEMBRANES
CELL PROLIFERATION
CONCANAVALIN A
DAYS LIVING RADIOISOTOPES
DISEASES
ELECTRON CAPTURE RADIOISOTOPES
GLYCOPROTEINS
HEMAGGLUTININS
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
IODINE 125
IODINE ISOTOPES
ISOTOPES
KERATIN
LECTINS
MEMBRANES
MICROORGANISMS
NEOPLASMS
NUCLEI
ODD-EVEN NUCLEI
ONCOGENIC VIRUSES
ORGANIC COMPOUNDS
PARASITES
PROTEINS
RADIOISOTOPES
SCLEROPROTEINS
TUMOR CELLS
VIRUSES