The application of synchrotron radiation to microprobe trace element analysis of biological samples
Conference
·
OSTI ID:5708785
An x-ray fluorescence microprobe (XRM) for trace element analysis is in operation at the National Synchrotron Light Source of Brookhaven National Laboratory. The properties of synchrotron radiation, in this case a continuous spectrum of x rays ranging up to 40 keV, make possible its uses as a microprobe beam with collimation or focusing with appropriate optical elements. Sensitivities in thin samples at 10 /times/ 10 /mu/m/sup 2/ spatial resolution is of the order of 10 fg using filtered, collimated ''white light'' and about 3 fg using a Kirkpatrick-Baez (KB) focusing system in thin standard foil. Results using a newly installed ellipsoidal mirror will be described. A discussion of standards for microprobe analysis is included. Examples of applications to the life sciences are described. These include the mapping of trace element distributions in thin tissue samples and examples of transmission and fluorescence microtomography. Synchrotron radiation microprobes complement ion and electron beam microprobes and the differences between the methods are considered. 23 figs., 4 tabs.
- Research Organization:
- Brookhaven National Lab., Upton, NY (USA)
- DOE Contract Number:
- AC02-76CH00016
- OSTI ID:
- 5708785
- Report Number(s):
- BNL-43048; CONF-8906161-6; ON: DE89017622
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400102* -- Chemical & Spectral Procedures
550200 -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ACCELERATORS
BIOLOGICAL MATERIALS
BREMSSTRAHLUNG
CHEMICAL ANALYSIS
CYCLIC ACCELERATORS
ELECTROMAGNETIC RADIATION
MATERIALS
MEASURING INSTRUMENTS
NONDESTRUCTIVE ANALYSIS
NSLS
PROBES
QUANTITATIVE CHEMICAL ANALYSIS
RADIATION SOURCES
RADIATIONS
SYNCHROTRON RADIATION
SYNCHROTRON RADIATION SOURCES
SYNCHROTRONS
TECHNOLOGY ASSESSMENT
TRACE AMOUNTS
X-RAY EMISSION ANALYSIS
X-RAY FLUORESCENCE ANALYSIS
400102* -- Chemical & Spectral Procedures
550200 -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ACCELERATORS
BIOLOGICAL MATERIALS
BREMSSTRAHLUNG
CHEMICAL ANALYSIS
CYCLIC ACCELERATORS
ELECTROMAGNETIC RADIATION
MATERIALS
MEASURING INSTRUMENTS
NONDESTRUCTIVE ANALYSIS
NSLS
PROBES
QUANTITATIVE CHEMICAL ANALYSIS
RADIATION SOURCES
RADIATIONS
SYNCHROTRON RADIATION
SYNCHROTRON RADIATION SOURCES
SYNCHROTRONS
TECHNOLOGY ASSESSMENT
TRACE AMOUNTS
X-RAY EMISSION ANALYSIS
X-RAY FLUORESCENCE ANALYSIS