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Ultraviolet radiation induces changes in membrane metabolism of human keratinocytes in culture

Journal Article · · J. Invest. Dermatol.; (United States)
; ; ; ;

Human keratinocytes in culture were prelabeled with (/sup 3/H)arachidonic acid (AA) and then exposed to ultraviolet B radiation. Irradiated cells released labeled AA metabolites into media in a dose-dependent manner when compared to sham-irradiated cells. The response began immediately and continued for 24 h. Extracts from media were examined by high-performance liquid chromatography for identification of specific AA metabolites. Irradiated cells were stimulated to produce prostaglandin-like material (PGE2 and PGF2 alpha). These findings support the concept that the cell membrane of keratinocytes participates directly or indirectly in initiating the sunburn response. It is also felt that the metabolites formed following injury to the membrane are an integral component in the mediation of that response.

Research Organization:
Columbia Univ., NY
OSTI ID:
5708035
Journal Information:
J. Invest. Dermatol.; (United States), Journal Name: J. Invest. Dermatol.; (United States) Vol. 83:5; ISSN JIDEA
Country of Publication:
United States
Language:
English

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Related Subjects

560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMAL TISSUES
ARACHIDONIC ACID
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BODY
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CELL CULTURES
CELL MEMBRANES
CHEMICAL REACTIONS
CHROMATOGRAPHY
DOSE-RESPONSE RELATIONSHIPS
ELECTROMAGNETIC RADIATION
EPIDERMIS
EPITHELIUM
FAR ULTRAVIOLET RADIATION
KERATIN
LABELLING
MEMBRANES
METABOLISM
METABOLITES
MONOCARBOXYLIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PHOTOCHEMICAL REACTIONS
PROSTAGLANDINS
PROTEINS
RADIATION EFFECTS
RADIATIONS
SCLEROPROTEINS
SEPARATION PROCESSES
SKIN
TISSUES
ULTRAVIOLET RADIATION