Physicochemical inactivation of Lassa, Ebola, and Marburg viruses and effect on clinical laboratory analyses

Journal Article · Sat Sep 01 00:00:00 EDT 1984 · J. Clin. Microbiol.; (United States)

OSTI ID:5707653

Mitchell, S W; McCormick, J B

Clinical specimens from patients infected with Lassa, Ebola, or Marburg virus may present a serious biohazard to laboratory workers. The authors have examined the effects of heat, alteration of pH, and gamma radiation on these viruses in human blood and on the electrolytes, enzymes, and coagulation factors measured in laboratory tests that are important in the care of an infected patient. Heating serum at 60 degrees C for 1 h reduced high titers of these viruses to noninfectious levels without altering the serum levels of glucose, blood urea nitrogen, and electrolytes. Dilution of blood in 3% acetic acid, diluent for a leukocyte count, inactivated all of these viruses. All of the methods tested for viral inactivation markedly altered certain serum proteins, making these methods unsuitable for samples that are to be tested for certain enzyme levels and coagulation factors.

• Research Organization: Centers for Disease Control, Atlanta, GA
• OSTI ID: 5707653
• Journal Information: J. Clin. Microbiol.; (United States), Journal Name: J. Clin. Microbiol.; (United States) Vol. 20:3; ISSN JCMID
• Country of Publication: United States
• Language: English

Related Subjects

560133* -- Radiation Effects on Microorganisms-- Radiosterilization of Medical Supplies-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BIOLOGICAL MATERIALS
BLOOD
BODY FLUIDS
ELECTROMAGNETIC RADIATION
GAMMA RADIATION
INACTIVATION
INFECTIVITY
IONIZING RADIATIONS
MATERIALS
MICROORGANISMS
PARASITES
PATIENTS
PH VALUE
RADIATIONS
RADIOSTERILIZATION
SAMPLE PREPARATION
STERILIZATION
TEMPERATURE EFFECTS
VIRUSES