Solid-phase radioimmunoassay for bound anti-platelet antibody. Studies on 45 patients with autoimmune platelet disorders. [/sup 125/I, PBI]

Journal Article · Mon Oct 01 04:00:00 EDT 1979 · J. Lab. Clin. Med.; (United States)

OSTI ID:5707435

Hymes, K; Shulman, S; Karpatkin, S

A highly sensitive, solid-phase radioimmunoassay has been developed for detecting platelet-bound immunoglobulin, which employs /sup 125/I-staphylococcal protein A. The assay detects platelet IgG at the picogram level, which is 10- to 50-fold more sensitive than currently available procedures. It is relatively simple and can be performed on frozen extracts from as little as 2 x 10/sup 5/ platelets. Washed platelets are frozen, thawed, sonicated, and centrifuged at 20,000 x g. The supernatant, which contains 7S IgG, is applied in serial dilution to the wells of a plastic microliter plate capable of adsorbing protein. Commercial rabbit anti-human IgG is added and then sandwiched to /sup 125/I-staphylococcal protein A. The wells are removed from the plate and assayed for radioactivity. Thirty-two of 35 thrombocytopenic patients (92%) with ATP had platelet IgG values greater than those of controls by 2 S.D. and averaged 150 +- 145 (S.D.) ng/10/sup 6/ platelets; 11 healthy controls, 11.4 +- 7.4 ng; five thrombocytopenic controls, 9.7 +- 13 ng. The platelet count (X) correlated inversely with IgG/platelet (Y) according to the equation: Log Y = -0.66 log X + 4.8; r = -0.71, p < 0.001. Elevated platelet IgG levels were also found in 5 ATP patients in apparent remission; two of four had evidence for compensated thrombocytolysis (increased megathrombocytes). Elevated platelet IgG levels were also found in six patients with thrombocytopenia secondary to lymphoproliferative disorders.

• Research Organization: New York Univ. Medical School, NY
• OSTI ID: 5707435
• Journal Information: J. Lab. Clin. Med.; (United States), Journal Name: J. Lab. Clin. Med.; (United States) Vol. 94:4; ISSN JLCMA
• Country of Publication: United States
• Language: English

Related Subjects

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59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
ANTIGEN-ANTIBODY REACTIONS
BACTERIA
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BLOOD PLATELETS
BODY FLUIDS
CARDIOVASCULAR DISEASES
CPB
DATA
DATA FORMS
DAYS LIVING RADIOISOTOPES
DIAGNOSIS
DISEASES
ELECTRON CAPTURE RADIOISOTOPES
EXPERIMENTAL DATA
GLOBULINS
HEMIC DISEASES
IMMUNE SERUMS
IMMUNOGLOBULINS
INFORMATION
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOLATED VALUES
ISOTOPE APPLICATIONS
ISOTOPES
MICROORGANISMS
NUCLEI
NUMERICAL DATA
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC IODINE COMPOUNDS
PBI
PROTEINS
PURPURA
RADIOASSAY
RADIOIMMUNOASSAY
RADIOISOTOPES
SENSITIVITY
STAPHYLOCOCCUS
TRACER TECHNIQUES