Chlorine-35 nuclear magnetic resonance study of zinc-nucleotide triphosphate complexes

Journal Article · Thu Dec 27 00:00:00 EST 1979 · J. Phys. Chem.; (United States)

DOI:https://doi.org/10.1021/j100489a029· OSTI ID:5707195

Happe, J A; Ward, R L

The interaction of Zn(II) with nucleotide triphosphates has been studied by /sup 35/Cl NMR and by /sup 1/H NMR. In 0.5 M NaCl solutions, enhanced /sup 35/Cl relaxation is produced by the metal ion in a Zn(II)-nucleotide triphosphate complex, Zn(NTP). In equimolar metal-ligand solutions, the adenosine triphosphate complex, Zn(ATP)/sup 2 -/, produces about twice as much /sup 35/Cl relaxation as do the inosine triphoshate or uridine triphosphate complexes, Zn(ITP)/sup 2 -/ and Zn(UTP)/sup 2 -/. /sup 35/Cl relaxation by the cytidine triphosphate complex, Zn(CTP)/sup 2 -/, is intermediate. In each case, the enhanced /sup 35/Cl relaxation found, relative to the Zn/sup 2 +/(aq) value, is removed by interaction with excess nucleotide. In the presence of excess Zn(II) ions, purine nucleotides form complexes which have high /sup 35/Cl molar relaxivity metal ion environments. These observations are discussed in terms of possible structures which could lead to such relaxation environments.

• Research Organization: Univ. of California, Livermore
• DOE Contract Number: W-7405-ENG-48
• OSTI ID: 5707195
• Journal Information: J. Phys. Chem.; (United States), Vol. 83:26
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
NUCLEOTIDES
CHEMICAL REACTIONS
PHOSPHATES
ZINC COMPLEXES
EXPERIMENTAL DATA
ISOLATED VALUES
NUCLEAR MAGNETIC RESONANCE
COMPLEXES
DATA
DATA FORMS
INFORMATION
MAGNETIC RESONANCE
NUMERICAL DATA
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
PHOSPHORUS COMPOUNDS
RESONANCE
550201* - Biochemistry- Tracer Techniques
550200 - Biochemistry