Accumulation of erythrocyte nucleotides and their pattern in lead workers

Journal Article · · Archives of Environmental Health; (USA)

DOI:https://doi.org/10.1080/00039896.1990.10118745· OSTI ID:5799049

Sakai, Tadashi; Araki, Takaharu; Ushio, Koichi ^[1]

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1. Tokyo Labor Accident Hospital (Japan)

Nucleotides in erythrocytes of lead-exposed subjects were analyzed by high-performance liquid chromatography (HPLC). Most of the pyrimidine levels correlated well with blood lead concentrations (Pb-B) pyrimidine 5{prime}-nucleotidase (P5N) activity. Highly significant correlations were found between Pb-B and uridine 5{prime}-diphosphate-glucose (UDPG), cytidine 5{prime}-triphosphate (CTP), or CDP-choline (CDPC). The levels of these compounds were sharply elevated when P5N activity was reduced to levels less than 7{mu}mole/ h{center dot}g hemoglobin (Hb), which corresponded to a Pb-B of 60 {mu}g/100 g. Therefore, concentration of these nucleotides may provide a useful index of lead poisoning. Adenosine 5{prime}-triphosphate (ATP) concentrations were correlated negatively with Pb-B, whereas adenosine 5{prime}-monophosphate (AMP) concentrations were correlated positively with Pb-B. These results suggest that lead affects not only pyrimidine nucleotide metabolism but also purine nucleotide metabolism (energy production system).

• OSTI ID: 5799049
• Journal Information: Archives of Environmental Health; (USA), Vol. 45:5; ISSN 0003-9896
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
59 BASIC BIOLOGICAL SCIENCES
LEAD
BIOLOGICAL EFFECTS
HEALTH HAZARDS
AMP
ATP
BLOOD SERUM
HEMOGLOBIN
LIQUID COLUMN CHROMATOGRAPHY
MAN
MONITORING
NUCLEOTIDES
PURINES
PYRIMIDINES
ANIMALS
AROMATICS
AZAARENES
AZINES
CARBOXYLIC ACIDS
CHROMATOGRAPHY
ELEMENTS
GLOBINS
HAZARDS
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
MAMMALS
METALS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PIGMENTS
PORPHYRINS
PRIMATES
PROTEINS
SEPARATION PROCESSES
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology
550900 - Pathology