Kinetics and sites of sequestration of indium 111-labeled human platelets during cardiopulmonary bypass

Journal Article · Mon Jun 01 04:00:00 EDT 1981 · J. Thorac. Cardiovasc. Surg.; (United States)

OSTI ID:5706782

Hope, A F; Heyns, A D; Loetter, M G; van Reenen, O R; de Kock, F; Badenhorst, P N; Pieters, H; Kotze, H; Meyer, J M; Minnaar, P C

A new approach for the study of the kinetics and quantification of the in vivo and ex vivo sites of sequestration of platelets during cardiopulmonary bypass (CPB) is described. Autologous platelets of four patients were labeled with /sup 111/In-oxine and reinfused on the day prior to CPB for coronary artery bypass grafting. Changes in blood /sup 111/In-labeled platelet radioactivity and blood platelet counts were monitored during the operation. In vivo /sup 111/In-labeled platelet redistribution was quantified with a scintillation camera and a computer-assisted imaging system before and after CPB. Sequestration of /sup 111/In-labeled platelets in the bubble oxygenator was measured. /sup 111/In-labeled platelet activity in the blood decreased by 46% +/- 5% within 5 minutes of CPB, but this decrease was mostly due to hemodilution; the true loss of platelets from the circulation was 13% +/- 4%. Intraoperatively, whole body /sup 111/In activity decreased by oxygenator 10.8% +/- 1.3% of administered platelets were sequestered, especially in the innermost active layers of the defoaming mesh of the bubble oxygenator. Mean survival time of circulating platelets was 58 +/- 8 hours and fitted an exponential function best. The bleeding time increased to 40 minutes during operation and returned to normal within 24 hours. During operation /sup 111/In-labeled platelets accumulated somewhat in the liver (10.7%) but not in the spleen, thorax, or head. In the 48 hours after operation, platelets were sequestered mainly in the liver. The scintillation camera with computer-assisted imaging allows in vivo quantitative studies of platelet kinetics of a type which has not been possible with previous techniques.

• OSTI ID: 5706782
• Journal Information: J. Thorac. Cardiovasc. Surg.; (United States), Journal Name: J. Thorac. Cardiovasc. Surg.; (United States) Vol. 81:6; ISSN JTCSA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ARTERIES
BETA DECAY RADIOISOTOPES
BIOCHEMISTRY
BIOLOGICAL LOCALIZATION
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BLOOD COAGULATION
BLOOD COUNT
BLOOD PLATELETS
BLOOD VESSELS
BODY
BODY FLUIDS
CARDIOVASCULAR SYSTEM
CHEMISTRY
CORONARIES
COUNTING TECHNIQUES
DAYS LIVING RADIOISOTOPES
DIAGNOSTIC TECHNIQUES
DIGESTIVE SYSTEM
DYNAMIC FUNCTION STUDIES
ELECTRON CAPTURE RADIOISOTOPES
GLANDS
GRAFTS
INDIUM 111
INDIUM ISOTOPES
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LIVER
MATERIALS
MINUTES LIVING RADIOISOTOPES
NUCLEI
ODD-EVEN NUCLEI
ORGANS
PATIENTS
RADIOISOTOPE SCANNING
RADIOISOTOPES
SCINTISCANNING
TRANSPLANTS