Investigation of the role of endogenous serotonin in the mechanism of action of radioprotective agents. Report 1. Role of endogenous serotonin in enhancing radioresistance of different types of cells treated with. beta. -mercaptoethylamine. [X rays; bone marrow cells; Ehrlich ascites tumor cells; Escherichia coli]

Journal Article · Sun Jan 01 04:00:00 EST 1978 · Radiobiology (USSR) (Engl. Transl.); (United States)

OSTI ID:5705865

Konstantinova, M M; Nekrasova, I V; Smirnova, I B; Gusareva, E V; Dontsova, G V; Manukhin, B N

The involvement of endogenous serotonin in expression of the radiomodifying effect of ..beta..-mercaptoethylamine was studied. Determination was made of the radiosensitivity of mouse bone marrow cells, Ehrlich's ascites carcinoma and E. coli B bacteria. The bone marrow suspension was exposed to x rays in vitro in a dosage of 150 R. Ehrlich's ascites tumor (EAT) cells were exposed once to x rays in a dosage of 700 R. A day-old culture of E. coli B bacteria, containing 1-5.10/sup 7/ cells/ml saline, was exposed to x rays in a dosage of 5 kR. Serotonin was assayed in intact cells and cells treated with MEA under conditions that were analogous to those used in the experiments with radiation. The data indicate that MEA enhances the radioresistance of all types of cells tested, regardless of dosage of agent and criterion selected to assess the radiation lesions. At the same time, the changes in endogenous serotonin content under the influence of MEA do not present such similarity. A comparison of the effect of MEA on radioresistance of cells to level of endogenous serotonin in them at the time of treatment shows that there is no correlation between these parameters. (ERB)

• Research Organization: Inst. of Developmental Biology, Moscow, USSR
• OSTI ID: 5705865
• Journal Information: Radiobiology (USSR) (Engl. Transl.); (United States), Journal Name: Radiobiology (USSR) (Engl. Transl.); (United States) Vol. 18:2; ISSN RADBA
• Country of Publication: United States
• Language: English

Related Subjects

560121* -- Radiation Effects on Cells-- External Source-- (-1987)
560131 -- Radiation Effects on Microorganisms-- Basic Studies-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINES
ANIMAL CELLS
ANIMALS
ASCITES TUMOR CELLS
AZOLES
BACTERIA
BONE MARROW CELLS
CONNECTIVE TISSUE CELLS
CORRELATIONS
DATA
DATA FORMS
DISEASES
DISPERSIONS
DRUGS
EHRLICH ASCITES TUMOR
ELECTROMAGNETIC RADIATION
ESCHERICHIA COLI
EXPERIMENTAL DATA
EXPERIMENTAL NEOPLASMS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
IN VITRO
INDOLES
INFORMATION
IONIZING RADIATIONS
IRRADIATION
ISOLATED VALUES
MAMMALS
MEA
MICE
MICROORGANISMS
NEOPLASMS
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PYRROLES
QUANTITY RATIO
RADIATIONS
RADIOPROTECTIVE SUBSTANCES
RADIOSENSITIVITY
RADIOSENSITIVITY EFFECTS
RESPONSE MODIFYING FACTORS
RODENTS
SEROTONIN
SOMATIC CELLS
SUSPENSIONS
SYMPATHOMIMETICS
THIOLS
TRYPTAMINES
TUMOR CELLS
VERTEBRATES
X RADIATION