Cloning, molecular characterization, and chromosomal assignment of a gene encoding a second D sub 1 dopamine receptor subtype: Differential expression pattern in rat brain compared with the D sup 1A receptor

Journal Article · Sun Sep 01 00:00:00 EDT 1991 · Proceedings of the National Academy of Sciences of the United States of America; (United States)

DOI:https://doi.org/10.1073/pnas.88.17.7491· OSTI ID:5703155

Tiberi, M; Jarvie, K R; Silvia, C; Falardeau, P; Gingrich, J A; Godinot, N; Bertrand, L; Fremeau, Jr, R T; Caron, M G ^[1]; Yang-Feng, T L ^[2]

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1. Duke Univ., Durham, NC (United States)
2. Yale Univ., New Haven, CT (United States)

Multiple D{sub 1} dopaminergic receptor subtypes have been postulated on the basis of pharmacological, biochemical, and genetic studies. The authors describe the isolation and characterization of a rat gene encoding a dopamine receptor that is structurally and functionally similar to the D{sub 1} dopamine receptor. The coding region, which is intronless, encodes a protein of 475 amino acids (M{sub r} 52,834) with structural features that are consistent with receptors coupled to guanine nucleotide-binding regulatory proteins. The expressed protein binds dopaminergic ligands and mediates stimulation of adenylyl cyclase with pharmacological properties similar to those of the D{sub 1} dopamine receptor. The gene encoding the human homologue of this receptor subtype is located to the short arm of chromosome 4 (4p16.3), the same region as the Huntington disease gene. In striking contrast to the previously cloned D{sub 1} receptor, little or no mRNA for the receptor described here was observed in striatum, nucleus accumbens, olfactory tubercle, and frontal cortex. High levels of mRNA for this receptor were found in distinct layers of the hippocampus, the mammillary nuclei, and the anterior pretectal nuclei, brain regions that have been shown to exhibit little or no D{sub 1} dopamine receptor binding. On the basis of its properties the authors propose that this dopamine receptor subtype be called D{sub 1B}.

• OSTI ID: 5703155
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (United States), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (United States) Vol. 88:17; ISSN 0027-8424; ISSN PNASA
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
AMINES
ANIMALS
AROMATICS
AUTONOMIC NERVOUS SYSTEM AGENTS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
BRAIN
CARDIOTONICS
CARDIOVASCULAR AGENTS
CENTRAL NERVOUS SYSTEM
CHROMOSOMES
CLONING
DAYS LIVING RADIOISOTOPES
DISEASES
DISTRIBUTION
DNA HYBRIDIZATION
DNA-CLONING
DOPAMINE
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
ETIOLOGY
EVEN-ODD NUCLEI
GENES
GENETIC MAPPING
HEREDITARY DISEASES
HETEROCHROMOSOMES
HIPPOCAMPUS
HYBRIDIZATION
HYDROGEN COMPOUNDS
HYDROXY COMPOUNDS
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
IODINE 125
IODINE ISOTOPES
ISOTOPES
LIGHT NUCLEI
MAMMALS
MAPPING
MEMBRANE PROTEINS
MESSENGER-RNA
NERVOUS SYSTEM
NEUROREGULATORS
NUCLEI
NUCLEIC ACIDS
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PHENOLS
POLYPHENOLS
PROTEINS
RADIOISOTOPES
RATS
RECEPTORS
RNA
RODENTS
SULFUR 35
SULFUR ISOTOPES
SYMPATHOMIMETICS
TISSUE DISTRIBUTION
TRITIUM COMPOUNDS
VERTEBRATES