Specific mutagenesis of a chlorophyll-binding protein. Progress report.
During the first phase of the project regarding specific mutagenesis of the chlorophyll-binding protein CP47 in photosystem II (PS II) most of the time has been devoted to (1) establishment of an optimal procedure for the reintroduction of psbB (the gene encoding CP47) carrying a site-directed mutation into the experimental organism, the cyanobacterium Synechocystis sp. PCC 6803, (2) preparations for site-directed mutagenesis, and (3) creation and analysis of chimaeric spinach/cyanobacterial CP47 mutants of Synechocystis. In the coming year, psbB constructs with site-directed mutations in potential chlorophyll-binding regions of CP47 will be introduced into the Synechocystis genome, and site-directed mutants will be characterized according to procedures described in the original project description. In addition, analysis of chimaeric CP47 mutants will be continued.
- Research Organization:
- Arizona State Univ., Tempe, AZ (United States)
- Sponsoring Organization:
- DOE; USDOE, Washington, DC (United States)
- DOE Contract Number:
- FG02-89ER14031
- OSTI ID:
- 5701773
- Report Number(s):
- DOE/ER/14031-1; ON: DE92007015
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
BACTERIA
BIOTECHNOLOGY
CHLOROPHYLL-BINDING PROTEINS
COMPARATIVE EVALUATIONS
CYANOBACTERIA
DOCUMENT TYPES
ENERGY TRANSFER
ESCHERICHIA COLI
EVALUATION
FOOD
GENETIC ENGINEERING
MAGNOLIOPHYTA
MAGNOLIOPSIDA
MICROORGANISMS
MUTAGENESIS
MUTATIONS
ORGANIC COMPOUNDS
PHOTOSYNTHETIC REACTION CENTERS
PLANTS
PROGRESS REPORT
PROTEINS
SPINACH
VEGETABLES