Specific mutagenesis of a chlorophyll-binding protein. [Synechocystis]
Chlorophyll, absorbing light energy for use in photosynthesis, is bound by specific proteins in thylakoid membranes such as CP47. His residues have been suggested to serve as chlorophyll ligands. To test the necessity of His residues in CP47, specific His residues have been altered to Tyr or Asn, and the effects of these mutations are now studied in vivo in the cyanobacterium Synechocystis sp. PCC 6803. Most mutations lead to a decreased photoautotrophic growth rate of the cyanobacterium, due in part to a decreased stability of the photosystem 2 complex. The effective relative antenna size of photosystem 2 in the various CP47 mutants is now being measured. The CP47 protein may also be involved in events at the donor side of photosystem 2. We have identified a region which can be deleted without obvious ill effects, and a domain which appears crucial for photosystem 2 structure and function. Residues of particular importance will be defined by further mutagenesis experiments. 1 fig., 1 tab.
- Research Organization:
- Arizona State Univ., Tempe, AZ (United States). Dept. of Botany
- Sponsoring Organization:
- DOE; USDOE, Washington, DC (United States)
- DOE Contract Number:
- FG02-89ER14031
- OSTI ID:
- 5671692
- Report Number(s):
- DOE/ER/14031-3; ON: DE91014813
- Country of Publication:
- United States
- Language:
- English
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550400 -- Genetics
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59 BASIC BIOLOGICAL SCIENCES
AMIDES
AMINO ACID SEQUENCE
AMINO ACIDS
ASPARAGINE
CARBOXYLIC ACIDS
CHEMICAL REACTIONS
CHLOROPHYLL
CHLOROPHYLL-BINDING PROTEINS
CYANOBACTERIA
DOCUMENT TYPES
GENES
GROWTH
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HYDROXY ACIDS
KINETICS
MEMBRANES
MICROORGANISMS
MOLECULAR STRUCTURE
MUTAGENESIS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHOTOCHEMICAL REACTIONS
PHOTOSYNTHESIS
PHOTOSYNTHETIC MEMBRANES
PHOTOSYNTHETIC REACTION CENTERS
PHYTOCHROMES
PIGMENTS
PORPHYRINS
PROGRESS REPORT
PROTEIN ENGINEERING
PROTEIN STRUCTURE
PROTEINS
STRUCTURE-ACTIVITY RELATIONSHIPS
SYNTHESIS
TYROSINE