Protein kinase C and regulation of the local competence of Xenopus ectoderm

Journal Article · Fri Feb 01 04:00:00 EST 1991 · Science (Washington, D.C.); (United States)

DOI:https://doi.org/10.1126/science.1990433· OSTI ID:5696760

Otte, A P; Kramer, I M; Durston, A J ^[1]

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1. Netherlands Inst. for Developmental Biology, Utrecht (Netherlands)

The limited competence of embryonic tissue to respond to an inductive signal has an essential, regulatory function in embryonic induction. The molecular basis for the competence of Xenopus ectoderm to differentiate into neural tissue was investigated. Dorsal mesoderm or 12-O-tetradecanoyl phorbol-13-acetate (TPA) caused in vivo activation of protein kinase C (PKC) and neural differentiation mainly in dorsal ectoderm and to a lesser extent in ventral ectoderm. These data correlate with the observations that PKC preparations from dorsal and ventral ectoderm differ, the dorsal PKC preparation being more susceptible to activation by TPA and diolein than is the ventral PKC preparation. Monoclonal antibodies against the bovine PKC {alpha} plus {beta} or {gamma} isozymes immunostained dorsal and ventral ectoderm, respectively, which suggests different localizations of PKC isozymes. These results suggest that PKC participates in the establishment of embryonic competence.

• OSTI ID: 5696760
• Journal Information: Science (Washington, D.C.); (United States), Journal Name: Science (Washington, D.C.); (United States) Vol. 251:4993; ISSN SCIEA; ISSN 0036-8075
• Country of Publication: United States
• Language: English

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63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
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ANIMALS
ANTIBODIES
AQUATIC ORGANISMS
BIOLOGICAL EFFECTS
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PHOSPHOTRANSFERASES
PROTEINS
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VERTEBRATES