NSrp70 is significant for embryonic growth and development, being a crucial factor for gastrulation and mesoderm induction
- ABRC, CMRI, School of Life Sciences, BK21 Plus KNU Creative BioResearch Group, Kyungpook National University, Daegu 41566 (Korea, Republic of)
- Department of Anatomy, College of Medicine, Kyungpook National University, Daegu 41944 (Korea, Republic of)
- Department of Laboratory Medicine, School of Medicine, Wonkwang University, Iksan 54538 (Korea, Republic of)
NSrp70 (nuclear speckle-related protein 70), a recently discovered protein and it belongs to the serine/arginine (SR) rich related protein family. NSrp70 is recognized as an important splicing factor comprising RNA recognition motif (RRM) and arginine/serine (RS)-like regions at the N- and C-terminus respectively, along with two coiled coil domains at each terminus. However, other functions of NSrp70 remain unelucidated. In this study, we investigated the role of NSrp70 in Xenopus embryogenesis and found that its maternal expression plays a critical role in embryonic development. Knockdown of NSrp70 resulted in dramatic reduction in the length of developing tadpoles and mild to severe malformation in Xenopus embryos. In addition, knockdown of NSrp70 resulted in an extremely short axis by blocking gastrulation and convergent extension. Further, animal cap assays along with activin A treatment revealed that NSrp70 is an essential factor for dorsal mesoderm induction as knockdown of NSrp70 caused a dramatic down-regulation of dorsal mesoderm specific genes and its loss significantly shortened the elongation region of animal caps. In conclusion, NSrp70 is crucial for early embryonic development, influencing gastrulation and mesoderm induction. - Highlights: • NSrp70 is required for proper length and dorso-anterior structures. • NSrp70 is essential for gastrulation movement during embryogenesis. • NSrp70 is crucial component for dorsal mesoderm induction.
- OSTI ID:
- 22696642
- Journal Information:
- Biochemical and Biophysical Research Communications, Vol. 479, Issue 2; Other Information: Copyright (c) 2016 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
- Country of Publication:
- United States
- Language:
- English
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