Elastolytic activity in the lungs of rats exposed to cadmium aerosolization

Journal Article · Fri Oct 01 00:00:00 EDT 1982 · Environ. Res.; (United States)

DOI:https://doi.org/10.1016/0013-9351(82)90010-X· OSTI ID:5695838

Padmanabhan, R V ^[1]; Gudapaty, S R; Liener, I E; Hoidal, J R

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1. Univ. of Minnesota, St. Paul

Rats were exposed for 1 hr per day for up to 35 days to an aerosol of 0.1% cadmium chloride. At periodic intervals, animals were sacrificed and their lungs lavaged. The lung lavage fluid was examined for polymorphonuclear leukocytes (PMN) and alveolar macrophages (AM). A portion of the cells of the lavage fluid was lysed, and the remainder of the cells were cultured. The lavage fluids, cell lysates, and conditioned media were assayed for elastolytic activity in the presence and absence of a peptide chloromethyl ketone and EDTA. Exposure to cadmium evoked a biphasic cellular response characterized by an initial influx (1-3 days) of PMN followed by a gradual increase in AM. This biphasic cellular response was accompanied by a shift in the type of elastolytic activity which was present in the lung lavage and its cellular components. The initial PMN phase was accompanied by the enhanced production of an elastase inhibited only by the peptide chloromethyl ketone, while the subsequent AM phase was associated with an elastase activity which was inhibited only by EDTA. The possible implication of these results with respect to the pathogenesis of emphysema is considered.

• OSTI ID: 5695838
• Journal Information: Environ. Res.; (United States), Vol. 29:1
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
CADMIUM CHLORIDES
BIOLOGICAL EFFECTS
HYDROLASES
ENZYME ACTIVITY
LEUKOCYTES
CELL PROLIFERATION
MACROPHAGES
AEROSOLS
INHALATION
LAVAGE
LUNGS
RATS
ANIMAL CELLS
ANIMALS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CADMIUM COMPOUNDS
CADMIUM HALIDES
CHLORIDES
CHLORINE COMPOUNDS
COLLOIDS
CONNECTIVE TISSUE CELLS
DISPERSIONS
ENZYMES
HALIDES
HALOGEN COMPOUNDS
INTAKE
MAMMALS
MATERIALS
ORGANS
PHAGOCYTES
RESPIRATORY SYSTEM
RODENTS
SOLS
SOMATIC CELLS
VERTEBRATES
560305* - Chemicals Metabolism & Toxicology- Vertebrates- (-1987)