Effects of metal ions on cyprinid fish immune response: In vitro effects of Zn2/sup +/ and Mn/sup 2+/ on the mitogenic response of carp pronephros lymphocytes
Lymphocytes from the pronephros of carp (Cyprinus carpio L) have been subjected to transformation by mitogens, concanavalin A (Con A), phytohemagglutinin (PHA), and lipopolysaccharides (LPS), with Zn or Mn at varying concentrations. Addition of Zn/sup 2+/ (10(-7) to 10(-3) M) to mitogen-stimulated T and B cells enhanced (/sup 3/H)thymidine incorporation. Addition of 10(-5) M Zn/sup 2+/ inhibited the response to Con A, PHA, and LPS. At this concentration, Zn was toxic. Addition of Mn2+ (10(-7) to 10(-3) M) to mitogen-stimulated lymphocytes enhanced (/sup 3/H)thymidine incorporation. This effect was observed with Con A- and PHA-stimulated lymphocytes, but not with LPS-stimulated lymphocytes. In contrast, addition of 10(-1) M Mn/sup 2+/ to lymphocyte cultures exerted an inhibitor on the response to Con A or to PHA, while the response to LPS was unaffected. Addition of 10(-1) M Mn/sup 2+/ to Con A- or PHA-stimulated cultures at different times after initiation of stimulation indicated that Mn/sup 2+/ was inhibitory only when it was added before the first 16 hr of cultures. The inhibition induced by 10(-1) M Mn2+ could be reversed by adding 2 mM CaCl/sub 2/ to the culture.
- Research Organization:
- Laboratoire d'Immunophysiologie generale et comparee, Limoges (France)
- OSTI ID:
- 5695797
- Journal Information:
- Ecotoxicol. Environ. Saf.; (United States), Vol. 17:2
- Country of Publication:
- United States
- Language:
- English
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59 BASIC BIOLOGICAL SCIENCES
FISHES
IMMUNE REACTIONS
LYMPHOCYTES
CELL PROLIFERATION
MAGNESIUM COMPOUNDS
TOXICITY
ZINC COMPOUNDS
CALCIUM
CATIONS
CONCANAVALIN
IN VITRO
LIPOPOLYSACCHARIDES
MITOGENS
MITOSIS
PHYTOHEMAGGLUTININ
THYMIDINE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
WATER POLLUTION
AGGLUTININS
ALKALINE EARTH METAL COMPOUNDS
ALKALINE EARTH METALS
ANIMAL CELLS
ANIMALS
ANTIBODIES
AQUATIC ORGANISMS
AZINES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOHYDRATES
CELL DIVISION
CHARGED PARTICLES
CONNECTIVE TISSUE CELLS
ELEMENTS
HEMAGGLUTININS
HETEROCYCLIC COMPOUNDS
IONS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LECTINS
LEUKOCYTES
LIPIDS
MATERIALS
METALS
MUCOPROTEINS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
POLLUTION
POLYSACCHARIDES
PROTEINS
PYRIMIDINES
RIBOSIDES
SACCHARIDES
SOMATIC CELLS
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology
550501 - Metabolism- Tracer Techniques