Glycine metabolism by Pseudomonas aeruginosa: hydrogen cyanide biosynthesis

Journal Article · Sun May 01 04:00:00 EDT 1977 · J. Bacteriol.; (United States)

OSTI ID:5690219

Castric, P A

Hydrogen cyanide (HCN) production by Pseudomonas aeruginosa in a synthetic medium is stimulated by the presence of glycine. Methionine enhances this stimulation but will not substitute for glycine as a stimulator of cyanogenesis. Threonine and phenylalanine are effective substitutes for glycine in the stimulation of HCN production. Glycine, threonine, and serine are good radioisotope precursors of HCN, but methionine and phenylalanine are not. Cell extracts of P. aeruginosa convert (/sup 14/C)threonine to (/sup 14/C)glycine. H14CN is produced with low dilution of label from either (1-/sup 14/C)glycine or (2-/sup 14/C)glycine, indicating a randomization of label either in the primary or secondary metabolism of glycine. When whole cells were fed (1,2-/sup 14/C)glycine, cyanide and bicarbonate were the only radioactive extracellular products observed.

• Research Organization: Duquesne Univ., Pittsburgh, PA
• OSTI ID: 5690219
• Journal Information: J. Bacteriol.; (United States), Journal Name: J. Bacteriol.; (United States) Vol. 2; ISSN JOBAA
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
BACTERIA
BIOSYNTHESIS
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
CYANIDES
DRUGS
GLYCINE
HYDROCYANIC ACID
HYDROGEN COMPOUNDS
HYDROXY ACIDS
INORGANIC ACIDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIPOTROPIC FACTORS
METABOLISM
METHIONINE
MICROORGANISMS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PHENYLALANINE
PSEUDOMONAS
SERINE
SYNTHESIS
THREONINE
TRACER TECHNIQUES