Method and apparatus for discrimination between scattered excitation radiation and low level fast decaying fluorescent radiation

Patent · Tue Apr 15 04:00:00 EST 1980

OSTI ID:5685224

Eneroth, P; Wladimiroff, W

A gated, pulsed laser spectrofluorometer is provided analyzing subpicogram amounts of fluorescent substance in sample volumes of less than 10 mu L. This is accomplished by repetitively exciting the fluorescent sample with a subnanosecond, intensive excitation radiation pulse and by gating a fluorescence radiation detector output signal so that detection starts after the excitation radiation pulse has decayed to a negligible intensity and ends when the fluorescence radiation detector output signal caused by the fluorescence emission from the sample has decayed to a level comparable to the electrical noise level of the electronic measuring apparatus connected to said fluorescence radiation detector.

• Assignee: IFI; EDB-82-059213
• Patent Number(s): US 4198567
• OSTI ID: 5685224
• Country of Publication: United States
• Language: English

Related Subjects

440300* -- Miscellaneous Instruments-- (-1989)
47 OTHER INSTRUMENTATION
CHEMICAL COMPOSITION
DECAY
DYE LASERS
ELECTRIC MEASURING INSTRUMENTS
ELECTRICAL EQUIPMENT
ELECTRONIC CIRCUITS
EMISSION
EMISSION SPECTROSCOPY
ENERGY-LEVEL TRANSITIONS
EQUIPMENT
FLUORESCENCE SPECTROSCOPY
GATING CIRCUITS
LASERS
LIQUID LASERS
MEASURING INSTRUMENTS
RADIATION DETECTORS
RADIATIONS
SPECTROSCOPY
STIMULATED EMISSION
WAVELENGTHS