Time-resolved Hyperspectral Fluorescence Spectroscopy using Frequency Modulated Excitation

Journal Article · Sun Jul 01 00:00:00 EDT 2012 · Journal of Applied Physics

DOI:https://doi.org/10.1063/1.4731247· OSTI ID:1055938

Neill, M

An intensity-modulated excitation light source is used together with a micro channel plate intensified CCD (ICCD) detector gated at a slightly different frequency to generate a beat frequency from a fluorescent sample. The addition of a spectrograph produces a hyperspectral time-resolved data product where the resulting beat frequency is detected with a low frame rate camera. Measuring the beat frequency of the spectrum as a function of time allows separation of the excited fluorescence from ambient constant light sources. The excitation and detector repetition rates are varied over a range of discrete frequencies, and the phase shift of the beat wave maps out the emission decay rate(s).

• Research Organization: Nevada Test Site (NTS), Mercury, NV (United States)
• Sponsoring Organization: USDOE National Nuclear Security Administration (NNSA)
• DOE Contract Number: DE-AC52-06NA25946
• OSTI ID: 1055938
• Report Number(s): DOE/NV/25946-1462
• Journal Information: Journal of Applied Physics, Vol. 112, Issue 1
• Country of Publication: United States
• Language: English

Related Subjects

46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND TECHNOLOGY
Laser-induced fluorescence
time-resolved spectroscopy
frequency modulated spectroscopy
frequency domain
beat frequency
remote sensing
spectroscopy
lifetime
hyperspectral measurements