Improved radioimmunoassay for creatine kinase isoenzymes in plasma

Journal Article · Sun Nov 01 00:00:00 EST 1981 · Clin. Chem. (Winston-Salem, N.C.); (United States)

OSTI ID:5682082

Ritter, C S; Mumm, S R; Roberts, R

We describe convenient and relatively rapid procedures for purifying creatine kinase isoenzymes MM, BB, and MB, and their use in an improved radioimmunoassay for creatine kinase isoenzymes in plasma. The modifications include use of: (a) BB with a specific activity of 400 kU/G, which can be labeled with a specific radioactivity of 20 Ci/g; (b) albumin-free purified MB as inhibitor; (c) antiserum to MB creatine kinase; and (d) a second-antibody technique that necessitates only a 15-min incubation. The radioimmunoassay for MB has a sensitivity of 0.2 ..mu..g/L (80 mU/L) and a CV of <5%. Plasma MB average 22 (SD 12) ..mu..g/L in 200 normal subjects; 24 (SD 12) ..mu..g/L in 200 patients with chest pain without infarction; and 23 (SD 7) ..mu..g/L in 43 patients with renal disease, whether measured before or after dialysis. Peak values for plasma MB averaged 191 (SD 86) ..mu..g/L in 325 patients with documented myocardial infarction; BB was negligible. Extensive clinical experience indicates the radioimmunoassay to be suitably rapid, highly sensitive, and reliable as a diagnostic assay for MB on plasma.

• Research Organization: Washington Univ. School of Medicine, St. Louis, MO
• OSTI ID: 5682082
• Journal Information: Clin. Chem. (Winston-Salem, N.C.); (United States), Vol. 27:11
• Country of Publication: United States
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
CARDIOVASCULAR DISEASES
DIAGNOSIS
RADIOIMMUNOASSAY
EVALUATION
UROGENITAL SYSTEM DISEASES
BLOOD PLASMA
ISOENZYMES
PATIENTS
BIOLOGICAL MATERIALS
BLOOD
BODY FLUIDS
DISEASES
ISOTOPE APPLICATIONS
MATERIALS
RADIOASSAY
TRACER TECHNIQUES
550601* - Medicine- Unsealed Radionuclides in Diagnostics