Pre-packed reverse phase columns for isolation of complex lipids synthesized from radioactive precursors
Journal Article
·
· J. Lipid Res.; (United States)
OSTI ID:5681702
Pre-packed reverse phase columns (Bond Elut) were used for the separation of complex lipids, such as phosphatidylcholine, cerebrosides, sulfatides, and gangliosides, from their respective water-soluble radioactive precursors after their in vitro biosynthesis. After an incubation in vitro, the entire reaction mixture is passed through the column, where complex lipids are retained and the hydrophilic radioactive precursors are washed away from the column. The retained lipids are then eluted with a more nonpolar organic solvent. The procedure is shown to be simpler and more efficient than the normally used Folch partitioning method or other procedures.
- Research Organization:
- Eunice Kennedy Shriver Center, Waltham, MA
- OSTI ID:
- 5681702
- Journal Information:
- J. Lipid Res.; (United States), Journal Name: J. Lipid Res.; (United States) Vol. 26:1; ISSN JLPRA
- Country of Publication:
- United States
- Language:
- English
Similar Records
Inhibition of cerebroside synthesis in the brains of mice treated with L-cycloserine
Covalent modification of hepatic microsomal lipids of rats by carbon tetrachloride
Triethyllead treatment of cultured brain cells. Effect on accumulation of radioactive precursors in galactolipids
Journal Article
·
Sun Mar 31 23:00:00 EST 1985
· J. Lipid Res.; (United States)
·
OSTI ID:5021344
Covalent modification of hepatic microsomal lipids of rats by carbon tetrachloride
Journal Article
·
Sat Jul 01 00:00:00 EDT 1989
· Molecular Toxicology; (USA)
·
OSTI ID:5978031
Triethyllead treatment of cultured brain cells. Effect on accumulation of radioactive precursors in galactolipids
Journal Article
·
Sat Jan 31 23:00:00 EST 1981
· Neurochem. Res.; (United States)
·
OSTI ID:5334280
Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BIOSYNTHESIS
BODY
BRAIN
CARBOHYDRATES
CARBON 14 COMPOUNDS
CENTRAL NERVOUS SYSTEM
CEREBROSIDES
CHROMATOGRAPHY
ESTERS
GANGLIOSIDES
GLYCOLIPIDS
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LECITHINS
LIPIDS
LIQUID COLUMN CHROMATOGRAPHY
MAMMALS
METABOLISM
NERVOUS SYSTEM
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANS
PHOSPHOLIPIDS
RATS
RODENTS
SACCHARIDES
SEPARATION PROCESSES
SULFUR ISOTOPES
SYNTHESIS
TRACER TECHNIQUES
VERTEBRATES
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BIOSYNTHESIS
BODY
BRAIN
CARBOHYDRATES
CARBON 14 COMPOUNDS
CENTRAL NERVOUS SYSTEM
CEREBROSIDES
CHROMATOGRAPHY
ESTERS
GANGLIOSIDES
GLYCOLIPIDS
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LECITHINS
LIPIDS
LIQUID COLUMN CHROMATOGRAPHY
MAMMALS
METABOLISM
NERVOUS SYSTEM
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANS
PHOSPHOLIPIDS
RATS
RODENTS
SACCHARIDES
SEPARATION PROCESSES
SULFUR ISOTOPES
SYNTHESIS
TRACER TECHNIQUES
VERTEBRATES