Kinetic deuterium isotope effects in glucocorticoid receptor activation
Activation and deactivation of the chick thymus glucocorticoid receptor protein was studied in ordinary and heavy water by DNA-cellulose binding of the tritiated triamcinolone acetonide-receptor complex. Activation was significantly slower in heavy water if it was promoted by incubation at elevated temperature in buffers of low ionic strength. In the presence of 300 mM KC1 or after separation from the low molecular weight cytosol constituents, the complex was activated at the same rate in both solvents. Deactivation (time dependent loss of DNA-binding capacity) was much faster in ordinary than in heavy water regardless of gel filtration or the presence of KC1. A model of receptor activation-deactivation was constructed on the basis of these data that accounts for the observed kinetic deuterium isotope effects and reveals some submolecular details of the process.
- Research Organization:
- Semmelweis Univ. Medical School, Budapest, Hungary
- OSTI ID:
- 5681666
- Journal Information:
- J. Receptor Res.; (United States), Journal Name: J. Receptor Res.; (United States) Vol. 4:1-6; ISSN JRERD
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
ADRENAL HORMONES
ANIMALS
BIOCHEMICAL REACTION KINETICS
BIRDS
BODY
CHICKENS
CORTICOSTEROIDS
DEUTERIUM
DNA
FOWL
GLUCOCORTICOIDS
HEAVY WATER
HORMONES
HYDROGEN COMPOUNDS
HYDROGEN ISOTOPES
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
ISOTOPE EFFECTS
ISOTOPES
KETONES
KINETICS
LABELLED COMPOUNDS
LIGHT NUCLEI
LYMPHATIC SYSTEM
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANS
OXYGEN COMPOUNDS
PREGNANES
REACTION KINETICS
RECEPTORS
STABLE ISOTOPES
STEROID HORMONES
STEROIDS
THYMUS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES
WATER