Characterization of a novel ACTH inducible cytochrome P-450 from rat adrenal microsomes

Conference · Mon Feb 26 04:00:00 EST 1990 · FASEB Journal (Federation of American Societies for Experimental Biology); (United States)

OSTI ID:5678396

Otto, S A; Marcus, C M; Jefcoate, C R ^[1]

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1. Univ. of Wisconsin, Madison (United States)

In rat adrenal cortex 7,12 dimethylbenz(a)anthracene (DMBA) causes massive necrosis that is dependent of ACTH. This is related to an ACTH inducible adrenal microsomal cytochrome P-450 that catalyzes hydrocarbon metabolism. Rat adrenal microsomes, catalyze the formation of DMBA 3,4 diol a precursor of the bay region reactive electrophile DMBA 3,4 diol 1,2 oxide. Both DMBA metabolism and a 57Kd protein have disappeared from microsomes 30 days after hypophysectomy, but are restored by 14 days treatment with ACTH. Dexamethasone which fully suppresses ACTH only partially suppresses this activity. The 57 Kd protein was partially purified to a single major band in one step from solubilized microsomes by h.p.l.c. chromatography using detergent elution from a novel column that mimics phospholipid membranes. This preparation exhibits a specific content of 2 nm P-450/mg protein and a turnover number of 1,500pm DMBA/nm P-450/minutes. A polyclonal antisera raised against this preparation provides a single western blot corresponding to the 57Kd ACTH sensitive protein. This antibody did not blot microsomal P-450 c21, nor did selected antibodies from known families react with this adrenal P-450 protein, suggesting substantial sequence differences from known P-450's.

• OSTI ID: 5678396
• Report Number(s): CONF-9004153--
• Conference Information: Journal Name: FASEB Journal (Federation of American Societies for Experimental Biology); (United States) Journal Volume: 4:3
• Country of Publication: United States
• Language: English

Related Subjects

550200 -- Biochemistry
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACTH
ADRENAL GLANDS
ANIMALS
AROMATICS
BENZANTHRACENE
BODY
CELL CONSTITUENTS
CONDENSED AROMATICS
ENDOCRINE GLANDS
ENZYME ACTIVITY
ENZYMES
ESTERS
GLANDS
HORMONES
HYDROCARBONS
IMMUNE REACTIONS
LIPIDS
MAMMALS
METABOLISM
METABOLITES
MICROSOMES
MIXED-FUNCTION OXIDASES
NECROSIS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANS
OXIDOREDUCTASES
OXYGENASES
PATHOLOGICAL CHANGES
PEPTIDE HORMONES
PHOSPHOLIPIDS
PITUITARY HORMONES
PROTEINS
RATS
RIBOSOMES
RODENTS
VERTEBRATES