Detection of DNA sequence polymorphisms in carcinogen metabolism genes by polymerase chain reaction
- National Inst. of Environmental Health Sciences, Research Triangle Park, NC (United States)
The glutathione transferase mu gene (GST1) and the debrisoquine hydroxylase gene (CYP2D6) are known to be polymorphic in the human population and have been associated with increased susceptibility to cancer. Smokers with low lymphocyte GST mu activity are at higher risk for lung cancer, while low debrisoquine hydroxylase activity has been correlated with lower risk for lung and bladder cancer. Phenotypic characterization of these polymorphisms by lymphocyte enzyme activity (GST) and urine metabolite ratios (debrisoquine) is cumbersome for population studies. Recent cloning and sequencing of the mutant alleles of these genes has allowed genotyping via the polymerase chain reaction (PCR). Advantages of PCR approaches are speed, technical simplicity, and minimal sample requirements. This article reviews the PCR-based methods for detection of genetic polymorphisms in human cancer susceptibility genes.
- OSTI ID:
- 5677828
- Journal Information:
- Environmental and Molecular Mutagenesis; (United States), Vol. 18:4; ISSN 0893-6692
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
CARCINOGENS
METABOLISM
RFLPS
DNA SEQUENCING
DNA POLYMERASES
ELECTROPHORESIS
GLUTATHIONE
HYDROXYLASES
TRANSFERASES
DRUGS
ENZYMES
NUCLEOTIDYLTRANSFERASES
ORGANIC COMPOUNDS
OXIDOREDUCTASES
PEPTIDES
PHOSPHORUS-GROUP TRANSFERASES
POLYMERASES
POLYPEPTIDES
PROTEINS
RADIOPROTECTIVE SUBSTANCES
STRUCTURAL CHEMICAL ANALYSIS
560300* - Chemicals Metabolism & Toxicology