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Title: Genetic polymorphism in three glutathione s-transferase genes and breast cancer risk

Abstract

The role of the glutathione S-transferase (GST) enzyme family is to detoxify environmental toxins and carcinogens and to protect organisms from their adverse effects, including cancer. The genes GSTM1, GSTP1, and GSTT1 code for three GSTs involved in the detoxification of carcinogens, such as polycyclic aromatic hydrocarbons (PAHs) and benzene. In humans, GSTM1 is deleted in about 50% of the population, GSTT1 is absent in about 20%, whereas the GSTP1 gene has a single base polymorphism resulting in an enzyme with reduced activity. Epidemiological studies indicate that GST polymorphisms increase the level of carcinogen-induced DNA damage and several studies have found a correlation of polymorphisms in one of the GST genes and an increased risk for certain cancers. We examined the role of polymorphisms in genes coding for these three GST enzymes in breast cancer. A breast tissue collection consisting of specimens of breast cancer patients and non-cancer controls was analyzed by polymerase chain reaction (PCR) for the presence or absence of the GSTM1 and GSTT1 genes and for GSTP1 single base polymorphism by PCR/RFLP. We found that GSTM1 and GSTT1 deletions occurred more frequently in cases than in controls, and GSTP1 polymorphism was more frequent in controls. The effectivemore » detoxifier (putative low-risk) genotype (defined as presence of both GSTM1 and GSTT1 genes and GSTP1 wild type) was less frequent in cases than controls (16% vs. 23%, respectively). The poor detoxifier (putative high-risk) genotype was more frequent in cases than controls. However, the sample size of this study was too small to provide conclusive results.« less

Authors:
; ; ; ; ;
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE Assistant Secretary for Energy Efficiency and Renewable Energy; the California Breast Cancer Research Program of the University of California, Grant Number 1RB-0429 (US)
OSTI Identifier:
799602
Report Number(s):
LBNL-50365
R&D Project: 43EM01; B& R 400403209; TRN: US200220%%640
DOE Contract Number:  
AC03-76SF00098
Resource Type:
Technical Report
Resource Relation:
Other Information: PBD: 1 Apr 2002
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 57; BENZENE; CARCINOGENS; DETOXIFICATION; DNA DAMAGES; ENZYMES; GENES; GENETICS; GENOTYPE; GLUTATHIONE; MAMMARY GLANDS; NEOPLASMS; PATIENTS; POLYCYCLIC AROMATIC HYDROCARBONS; POLYMERASE CHAIN REACTION; TOXINS

Citation Formats

Woldegiorgis, S., Ahmed, R.C., Zhen, Y., Erdmann, C.A., Russell, M.L., and Goth-Goldstein, R. Genetic polymorphism in three glutathione s-transferase genes and breast cancer risk. United States: N. p., 2002. Web. doi:10.2172/799602.
Woldegiorgis, S., Ahmed, R.C., Zhen, Y., Erdmann, C.A., Russell, M.L., & Goth-Goldstein, R. Genetic polymorphism in three glutathione s-transferase genes and breast cancer risk. United States. doi:10.2172/799602.
Woldegiorgis, S., Ahmed, R.C., Zhen, Y., Erdmann, C.A., Russell, M.L., and Goth-Goldstein, R. Mon . "Genetic polymorphism in three glutathione s-transferase genes and breast cancer risk". United States. doi:10.2172/799602. https://www.osti.gov/servlets/purl/799602.
@article{osti_799602,
title = {Genetic polymorphism in three glutathione s-transferase genes and breast cancer risk},
author = {Woldegiorgis, S. and Ahmed, R.C. and Zhen, Y. and Erdmann, C.A. and Russell, M.L. and Goth-Goldstein, R.},
abstractNote = {The role of the glutathione S-transferase (GST) enzyme family is to detoxify environmental toxins and carcinogens and to protect organisms from their adverse effects, including cancer. The genes GSTM1, GSTP1, and GSTT1 code for three GSTs involved in the detoxification of carcinogens, such as polycyclic aromatic hydrocarbons (PAHs) and benzene. In humans, GSTM1 is deleted in about 50% of the population, GSTT1 is absent in about 20%, whereas the GSTP1 gene has a single base polymorphism resulting in an enzyme with reduced activity. Epidemiological studies indicate that GST polymorphisms increase the level of carcinogen-induced DNA damage and several studies have found a correlation of polymorphisms in one of the GST genes and an increased risk for certain cancers. We examined the role of polymorphisms in genes coding for these three GST enzymes in breast cancer. A breast tissue collection consisting of specimens of breast cancer patients and non-cancer controls was analyzed by polymerase chain reaction (PCR) for the presence or absence of the GSTM1 and GSTT1 genes and for GSTP1 single base polymorphism by PCR/RFLP. We found that GSTM1 and GSTT1 deletions occurred more frequently in cases than in controls, and GSTP1 polymorphism was more frequent in controls. The effective detoxifier (putative low-risk) genotype (defined as presence of both GSTM1 and GSTT1 genes and GSTP1 wild type) was less frequent in cases than controls (16% vs. 23%, respectively). The poor detoxifier (putative high-risk) genotype was more frequent in cases than controls. However, the sample size of this study was too small to provide conclusive results.},
doi = {10.2172/799602},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Mon Apr 01 00:00:00 EST 2002},
month = {Mon Apr 01 00:00:00 EST 2002}
}

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