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Radiation-induced products of peptides and their enzymatic digestibility

Thesis/Dissertation ·
OSTI ID:5675646

Chemical characterization of radiation-induced products of peptides and proteins is essential for understanding the effect of ionizing radiation on peptides and proteins. Furthermore, peptides containing radiation-altered amino acid residues might not be completely digestible by proteolytic enzymes. In this work, small homopeptides of Ala, Phe and Met were chosen as model peptides. Lysozyme was used to investigate the effect of ionizing radiation on a small protein. All peptides and lysozyme were irradiated in diluted, oxygen free, N/sub 2/O-saturated aqueous solutions, using a /sup 60/Co-..gamma..-source. HPLC, capillary GC and GC-MS were applied to isolate and characterize the radiation-induced products. The enzymatic digestibility of the products was investigated using aminopeptidase M, leucine aminopeptidase, carboxypeptidase A and carboxypeptidase Y. It was found that irradiation of peptides examined in this work leads to racemization and alteration of amino acid residues and crosslinks between the peptide chains. In addition, it was established that exopeptidases act differently on radiation-induced dimers of peptides composed of aliphatic, aromatic and sulfur-containing amino acids.

Research Organization:
American Univ., Washington, DC
OSTI ID:
5675646
Country of Publication:
United States
Language:
English

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Related Subjects

38 RADIATION CHEMISTRY, RADIOCHEMISTRY, AND NUCLEAR CHEMISTRY
400600* -- Radiation Chemistry
ALANINES
AMINO ACIDS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOXYLIC ACIDS
CHEMICAL RADIATION EFFECTS
CHEMICAL REACTIONS
CHEMISTRY
CHROMATOGRAPHY
COBALT 60
COBALT ISOTOPES
DATA
DECOMPOSITION
DISSOLUTION
DISSOLVERS
DRUGS
ENZYMES
EXPERIMENTAL DATA
GLYCOSYL HYDROLASES
HYDROLASES
INFORMATION
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
LIPOTROPIC FACTORS
LIQUID COLUMN CHROMATOGRAPHY
LYSOZYME
MASS SPECTROSCOPY
METHIONINE
MINUTES LIVING RADIOISOTOPES
NUCLEI
NUMERICAL DATA
O-GLYCOSYL HYDROLASES
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PHENYLALANINE
POLYMERIZATION
RADIATION CHEMISTRY
RADIATION EFFECTS
RADIOISOTOPES
RADIOLYSIS
SEPARATION PROCESSES
SPECTROSCOPY
THIN-LAYER CHROMATOGRAPHY
YEARS LIVING RADIOISOTOPES