Characterization of a benzyladenine binding-site peptide isolated from a wheat cytokinin-binding protein: Sequence analysis and identification of a single affinity-labeled histidine residue by mass spectrometry
Journal Article
·
· Proceedings of the National Academy of Sciences of the United States of America; (USA)
- ARCO Plant Cell Research Institute, Dublin, CA (USA)
A wheat embryo cytokinin-binding protein was covalently modified with the radiolabeled photoaffinity ligand 2-azido-N{sup 6}-({sup 14}C)benzyladenine. A single labeled peptide was obtained after proteolytic digestion and isolation by reversed-phase and anion-exchange HPLC. Sequencing by classical Edman degradation identified 11 of the 12 residues but failed to identify the labeled amino acid. Analysis by laser photodissociation Fourier-transform mass spectrometry of 10 pmol of the peptide independently confirmed the Edman data and also demonstrated that the histidine residue nearest the C terminus (underlined) was modified by the reagent in the sequence Ala-Phe-Leu-Gln-Pro-Ser-His-His{und His}-Asp-Ala-Asp-Glu.
- OSTI ID:
- 5517444
- Journal Information:
- Proceedings of the National Academy of Sciences of the United States of America; (USA), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (USA) Vol. 85:16; ISSN 0027-8424; ISSN PNASA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADENINES
AMINES
AMINO ACID SEQUENCE
AMINO ACIDS
ANTIMETABOLITES
AROMATICS
AZAARENES
AZOLES
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
CEREALS
CHEMICAL REACTIONS
CHROMATOGRAPHY
CROSS-LINKING
DRUGS
GRAMINEAE
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HISTIDINE
IMIDAZOLES
ION EXCHANGE CHROMATOGRAPHY
KININS
LABELLED COMPOUNDS
LIGANDS
LILIOPSIDA
MAGNOLIOPHYTA
MASS SPECTROSCOPY
MOLECULAR STRUCTURE
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PEPTIDES
PLANTS
POLYMERIZATION
POLYPEPTIDES
PROTEINS
PURINES
SEPARATION PROCESSES
SPECTROSCOPY
WHEAT
59 BASIC BIOLOGICAL SCIENCES
ADENINES
AMINES
AMINO ACID SEQUENCE
AMINO ACIDS
ANTIMETABOLITES
AROMATICS
AZAARENES
AZOLES
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
CEREALS
CHEMICAL REACTIONS
CHROMATOGRAPHY
CROSS-LINKING
DRUGS
GRAMINEAE
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HISTIDINE
IMIDAZOLES
ION EXCHANGE CHROMATOGRAPHY
KININS
LABELLED COMPOUNDS
LIGANDS
LILIOPSIDA
MAGNOLIOPHYTA
MASS SPECTROSCOPY
MOLECULAR STRUCTURE
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PEPTIDES
PLANTS
POLYMERIZATION
POLYPEPTIDES
PROTEINS
PURINES
SEPARATION PROCESSES
SPECTROSCOPY
WHEAT