Fate of erythrocyte Cd-metallothionein in mice

Journal Article · Mon Mar 31 23:00:00 EST 1986 · Toxicol. Appl. Pharmacol.; (United States)

DOI:https://doi.org/10.1016/0041-008X(86)90296-6· OSTI ID:5674436

Tanaka, K; Min, K S; Ohyanagi, N; Onosaka, S; Fukuhara, C

Degradation of metallothionein (MT), which appears in erythrocytes following cadmium (Cd) administration, was investigated in mice. Cd-MT underwent only slight decomposition by hemolysate in an in vitro experiment unlike an 800g supernatant fraction of the liver homogenate. In an in vivo study, (/sup 3/H)diisopropylfluorophosphate was given to mice which had received /sup 109/CdCl2 to investigate the relationship between the decay of /sup 109/Cd-MT in the erythrocyte and the life span of the erythrocyte. A similar reduction pattern of radioactivity of /sup 109/Cd and /sup 3/H was observed. Erythrocytes containing /sup 109/Cd-MT obtained from mice preadministered with /sup 109/CdCl/sub 2/ was transfused to normal mice. The /sup 109/Cd radioactivity of erythrocytes decreased in a manner similar to Cd in erythrocytes of /sup 109/CdCl/sub 2/-administered mice. Contrary to this decrease of erythrocyte Cd in the transfused mice, Cd concentration of the spleen increased markedly. Cd increased also in the liver. These results indicate that erythrocyte MT degrades along with the erythrocyte. The Cd from this MT is deposited in the spleen and liver where blood cells are catabolized.

• Research Organization: Kobe-Gakuin Univ., Japan
• OSTI ID: 5674436
• Journal Information: Toxicol. Appl. Pharmacol.; (United States), Journal Name: Toxicol. Appl. Pharmacol.; (United States) Vol. 2; ISSN TXAPA
• Country of Publication: United States
• Language: English

Related Subjects

550501 -- Metabolism-- Tracer Techniques
560305* -- Chemicals Metabolism & Toxicology-- Vertebrates-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BETA DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CADMIUM
CADMIUM 109
CADMIUM ISOTOPES
DIGESTIVE SYSTEM
DISTRIBUTION
ELECTRON CAPTURE RADIOISOTOPES
ELEMENTS
ERYTHROCYTES
EVEN-ODD NUCLEI
GLANDS
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LIFE SPAN
LIVER
MAMMALS
MATERIALS
METABOLISM
METALLOPROTEINS
METALLOTHIONEIN
METALS
MICE
NUCLEI
ORGANIC COMPOUNDS
ORGANS
OXYGEN COMPOUNDS
PHOSPHATES
PHOSPHORUS COMPOUNDS
PROTEINS
RADIOISOTOPES
RODENTS
SPLEEN
TISSUE DISTRIBUTION
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES
YEARS LIVING RADIOISOTOPES