The oncogenic action of ionizing radiation on rat skin: Progress report, February 1, 1987-January 31, 1988

Technical Report · Thu Jan 01 04:00:00 EST 1987

OSTI ID:5672776

Burns, F J

The work outlined in this report includes: epidermal DNA strand breaks and radiation penetration; activation of oncogenes in radiation induced rat skin tumors; and rat skin carcinogenesis by neon ions. The effect of radiation penetration on DNA single strand breaks has been studied extensively in rat and mouse epidermis. The results show clearly that the number of strand breaks per unit dose in the epidermal DNA is reduced by 50% to 65% when the radiation penetration is reduced from 1.0 mm to 0.2 mm. This penetration effect on DNA strand breaks was not seen in mouse epidermal cell lines growing in plastic dishes. The results imply that DNA strand breakage in superficial cells is partially dependent on the radiation dose to underlying tissue. The phenomenon is not mediated by systemic interactions as it was observed in irradiated explanted skin. The oncogene activation pattern in the radiation-induced skin tumors was found to be tumor dependent. Either K-ras activation or c-myc amplification or both was observed in each tumor analyzed so far. Even benign fibromas exhibited c-myc amplification. The carcinogenicity of high penetration electrons (2.0 MeV) was determined in preparation for similar studies with a neon ion beam at the Berkeley Bevelac. The principal finding so far is a large excess of connective tissue tumors, fibromas (benign) and sarcomas (malignant). 59 refs., 1 tab.

• Research Organization: New York Univ., NY (USA). Dept. of Environmental Medicine
• DOE Contract Number: FG02-87ER60539
• OSTI ID: 5672776
• Report Number(s): DOE/ER/60539-3; ON: DE88002637
• Country of Publication: United States
• Language: English

Related Subjects

560151* -- Radiation Effects on Animals-- Man
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BODY
CARCINOGENESIS
CHEMICAL ACTIVATION
DNA
DOCUMENT TYPES
DOSE-RESPONSE RELATIONSHIPS
DOSES
GENES
MAMMALS
NUCLEIC ACIDS
ONCOGENES
ORGANIC COMPOUNDS
ORGANS
PATHOGENESIS
PENETRATION DEPTH
PROGRESS REPORT
RADIATION DOSES
RADIATION EFFECTS
RADIOINDUCTION
RATS
RESPONSE MODIFYING FACTORS
RODENTS
SKIN
VERTEBRATES